Validation Data Gallery

Filter: AllWBIF/ICC

WB analysis of HepG2 using 16497-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 16497-1-AP (RPL31 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 16497-1-AP
WB result of RPL31 antibody (16497-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-RPL31 transfected HepG2 cells.
WB analysis of NIH/3T3 using 16497-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16497-1-AP (RPL31 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of NIH/3T3 using 16497-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16497-1-AP (RPL31 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 16497-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 16497-1-AP (RPL31 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 16497-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 16497-1-AP (RPL31 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IF Staining of HepG2 using 16497-1-AP
Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 16497-1-AP (RPL31 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

RPL31 Polyclonal antibody

RPL31 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 16497-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

ribosomal protein L31, Large ribosomal subunit protein eL31, 60S ribosomal protein L31

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (3)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HepG2 cells, mouse liver tissue, NIH/3T3 cells
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 2 publications below
IF	See 1 publications below

Product Information

16497-1-AP targets RPL31 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	RPL31 fusion protein Ag9648 相同性解析による交差性が予測される生物種
Full Name	ribosomal protein L31
Calculated molecular weight	125 aa, 14 kDa
Observed molecular weight	14 kDa
GenBank accession number	BC017343
Gene Symbol	RPL31
Gene ID (NCBI)	6160
RRID	AB_2181772
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P62899
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

The mammalian ribosome comprises 79 ribosomal proteins and four rRNAs, which combine in equimolar ratios to form the small (40S) and large (60S) subunits. Ribosome proteins are a direct and critical target of the PI3K pathway in promoting growth.[PMID:15289434]. Ribosomal protein L31 gene is a component of the 60S large ribosomal subunit encoded by RPL31 gene, while ribosomal protein L31 (RPL31) is an important constituent of peptidyltransferase center [PMID:22714919].

Protocols

Product Specific Protocols
WB protocol for RPL31 antibody 16497-1-AP	Download protocol
IF protocol for RPL31 antibody 16497-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Curr Biol RPL10L Is Required for Male Meiotic Division by Compensating for RPL10 during Meiotic Sex Chromosome Inactivation in Mice. Authors - Long Jiang View Article
mouse	IF	J Med Genet Bi-allelic variants in chromatoid body protein TDRD6 cause spermiogenesis defects and severe oligoasthenoteratozoospermia in humans Authors - Rui Guo View Article
human	WB	iScience TIAR and FMRP shape pro-survival nascent proteome of leukemia cells in the bone marrow microenvironment Authors - Magdalena Wolczyk View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of HepG2 using 16497-1-AP

WB analysis of HepG2 using 16497-1-AP

WB analysis of NIH/3T3 using 16497-1-AP