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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RPL36 Polyclonal antibody

RPL36 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 15145-1-AP

Synonyms

ribosomal protein L36, Large ribosomal subunit protein eL36, 60S ribosomal protein L36

Validation Data Gallery

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  • Western Blot (WB) analysis of PC-12 cells using RPL36 Polyclonal antibody (15145-1-AP)

    WB analysis of PC-12 using 15145-1-AP

    PC-12 cells were subjected to SDS PAGE followed by western blot with 15145-1-AP (RPL36 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of BxPC-3 cells using RPL36 Polyclonal antibody (15145-1-AP)

    WB analysis of BxPC-3 using 15145-1-AP

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 15145-1-AP (RPL36 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using RPL36 Polyclonal antibody (15145-1-AP)

    WB analysis of HepG2 using 15145-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 15145-1-AP (RPL36 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using RPL36 Polyclonal antibody (15145-1-AP)

    WB analysis of HepG2 using 15145-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 15145-1-AP (RPL36 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using RPL36 Polyclonal antibody (15145-1-AP)

    WB analysis of NIH/3T3 using 15145-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 15145-1-AP (RPL36 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using RPL36 Polyclonal antibody (15145-1-AP)

    WB analysis of HeLa using 15145-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15145-1-AP (RPL36 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RPL36 Polyclonal antibody (15145-1-AP)

    IF Staining of HeLa using 15145-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using RPL36 antibody (15145-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

Positive WB detected inPC-12 cells, BxPC-3 cells, HeLa cells, HepG2 cells, NIH/3T3 cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

15145-1-AP targets RPL36 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RPL36 fusion protein Ag7437 相同性解析による交差性が予測される生物種
Full Name ribosomal protein L36
Calculated molecular weight 12 kDa
Observed molecular weight 12-14 kDa
GenBank accession numberBC003052
Gene symbol RPL36
Gene ID (NCBI) 25873
RRIDAB_2878111
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RPL36, also named as 60S ribosomal protein L36, is a 105 amino acid protein, which is a component of the large ribosomal subunit. RPL36 may be involved in the early stage of hepatocarcinogenesis, and it can be used as an independent and potential prognostic marker for resected HCC.

Protocols

Product Specific Protocols
WB protocol for RPL36 antibody 15145-1-APDownload protocol
IF protocol for RPL36 antibody 15145-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Cell Biol

METTL3 preferentially enhances non-m6A translation of epigenetic factors and promotes tumourigenesis

Authors - Xueju Wei
View Article
mouseWB

J Pineal Res

Amelioration of gamma irradiation-induced salivary gland damage in mice using melatonin

Authors - Jingming Ren
View Article