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KD/KO Validated

RPL39 Polyclonal antibody

Name: RPL39 Polyclonal antibody
Brand: Proteintech
SKU: 14990-1-AP

RPL39 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 14990-1-AP

Synonyms

ribosomal protein L39, Large ribosomal subunit protein eL39, eL39, 60S ribosomal protein L39

Validation Data Gallery

Filter:

AllWBIHCIF/ICC

WB analysis of HepG2 using 14990-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 14990-1-AP (RPL39 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of human liver using 14990-1-AP
human liver tissue were subjected to SDS PAGE followed by western blot with 14990-1-AP (RPL39 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IHC staining of human liver cancer using 14990-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer using 14990-1-AP (RPL39 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human liver cancer using 14990-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer using 14990-1-AP (RPL39 antibody) at dilution of 1:100 (under 40x lens).
IF Staining of HepG2 using 14990-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RPL39 antibody (14990-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Cat No. 14990-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HepG2 cells, human liver tissue
Positive IHC detected in	human liver cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 4 publications below
WB	See 5 publications below
IHC	See 2 publications below
IF	See 5 publications below
IP	See 1 publications below
FC	See 1 publications below

Product Information

14990-1-AP targets RPL39 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, mouse, zebrafish
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	RPL39 fusion protein Ag6975 相同性解析による交差性が予測される生物種
Full Name	ribosomal protein L39
Calculated molecular weight	6 kDa
Observed molecular weight	6 kDa
GenBank accession number	BC001019
Gene symbol	RPL39
Gene ID (NCBI)	6170
RRID	AB_2238670
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for RPL39 antibody 14990-1-AP	Download protocol
IHC protocol for RPL39 antibody 14990-1-AP	Download protocol
IF protocol for RPL39 antibody 14990-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Mol Cell Early Scanning of Nascent Polypeptides inside the Ribosomal Tunnel by NAC. Authors - Martin Gamerdinger View Article
human	FC	Transl Res Ribosomal proteins as distinct "passengers" of microvesicles: new semantics in myeloma and mesenchymal stem cells' communication. Authors - Mahmoud Dabbah View Article
zebrafish	IF	Elife In vivo proximity labeling identifies cardiomyocyte protein networks during zebrafish heart regeneration. Authors - Mira I Pronobis View Article
human	IF	Cell Discov Ribosomal RNA regulates chromosome clustering during mitosis. Authors - Kai Ma View Article
human	WB,IHC,IF	Front Immunol Identification and validation of a 17-gene signature to improve the survival prediction of gliomas Authors - Shiao Tong KD Validated View Article
mouse	WB,IP,IF	iScience Proteostasis regulated by testis-specific ribosomal protein RPL39L maintains mouse spermatogenesis. Authors - Qianxing Zou KO Validated View Article

製品

アプリケーション

cGMPグレード製品

サポート

カスタム対応

プロモーション

企業情報

抗体

免疫アッセイ

サイトカイン・増殖因子

ChromoTek VHH（Nanobody®）試薬

サポートツール・試薬

ゲノミクス試薬

研究分野

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RPL39 Polyclonal antibody

RPL39 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Validation Data Gallery

WB analysis of HepG2 using 14990-1-AP

WB analysis of human liver using 14990-1-AP

IHC staining of human liver cancer using 14990-1-AP

IHC staining of human liver cancer using 14990-1-AP

IF Staining of HepG2 using 14990-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Early Scanning of Nascent Polypeptides inside the Ribosomal Tunnel by NAC.

Ribosomal proteins as distinct "passengers" of microvesicles: new semantics in myeloma and mesenchymal stem cells' communication.

In vivo proximity labeling identifies cardiomyocyte protein networks during zebrafish heart regeneration.

Ribosomal RNA regulates chromosome clustering during mitosis.

Identification and validation of a 17-gene signature to improve the survival prediction of gliomas

Proteostasis regulated by testis-specific ribosomal protein RPL39L maintains mouse spermatogenesis.