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Ribosomal protein L4 Polyclonal antibody

Ribosomal protein L4 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, RIP, ELISA

Conjugate

Unconjugated

Cat no : 11302-1-AP

Synonyms

RPL4, RPL1, RPL 4, 60S ribosomal protein L4, 60S ribosomal protein L1


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Tested Applications

Positive WB detected inHeLa cells, rat skeletal muscle tissue, HepG2 cells, MCF-7 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11302-1-AP targets Ribosomal protein L4 in WB, IHC, IF/ICC, FC (Intra), IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rice, arabidopsis
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Ribosomal protein L4 fusion protein Ag1828 相同性解析による交差性が予測される生物種
Full Name ribosomal protein L4
Calculated molecular weight 48 kDa
Observed molecular weight 48 kDa
GenBank accession numberBC009888
Gene symbol Ribosomal protein L4
Gene ID (NCBI) 6124
RRIDAB_2181909
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

60S ribosomal protein L4 is encoded by RPL4 (or RPL1) gene. RPL4 is a component of 60S subunit of ribosome, and belongs to the L4E family. By interacting with c-Myb, RPL4 plays a important role in c-myc expression in response to growth factor and nutritional signals. Recently RPL4 was reported to be able to increase effeiciency of viral recoding sequence.

Protocols

Product Specific Protocols
WB protocol for Ribosomal protein L4 antibody 11302-1-APDownload protocol
IHC protocol for Ribosomal protein L4 antibody 11302-1-APDownload protocol
IF protocol for Ribosomal protein L4 antibody 11302-1-APDownload protocol
IP protocol for Ribosomal protein L4 antibody 11302-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Science

The Ccr4-Not complex monitors the translating ribosome for codon optimality.

Authors - Robert Buschauer
humanWB

Cell

Ribosome Collisions Trigger General Stress Responses to Regulate Cell Fate.

Authors - Colin Chih-Chien Wu
mouseIF

Cell Res

Pre-ribosomal RNA reorganizes DNA damage repair factors in nucleus during meiotic prophase and DNA damage response.

Authors - Xiaochen Gai
mouseWB

Mol Cell

RAPIDASH: Tag-free enrichment of ribosome-associated proteins reveals composition dynamics in embryonic tissue, cancer cells, and macrophages

Authors - Teodorus Theo Susanto
humanWB

Mol Cell

N6-Methyladenosine Guides mRNA Alternative Translation during Integrated Stress Response.

Authors - Jun Zhou
humanWB

Mol Cell

The G3BP1-Family-USP10 Deubiquitinase Complex Rescues Ubiquitinated 40S Subunits of Ribosomes Stalled in Translation from Lysosomal Degradation.

Authors - Cindy Meyer

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Anita (Verified Customer) (10-30-2020)

1:1000 dilution works for HeLa cells, not for C. elegans nematodes

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
Ribosomal protein L4 Antibody Western Blot validation (1:1000 dilution) in (Cat no:11302-1-AP)