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Ribosomal protein L4 Polyclonal antibody
Ribosomal protein L4 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (2)
Applications
WB, IHC, IF/ICC, FC (Intra), IP, RIP, ELISA
Conjugate
Unconjugated
Cat no : 11302-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, rat skeletal muscle tissue, HepG2 cells, MCF-7 cells |
Positive IP detected in | HeLa cells |
Positive IHC detected in | human lymphoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HeLa cells |
Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:10000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 1 publications below |
WB | See 33 publications below |
IF | See 2 publications below |
IP | See 2 publications below |
FC | See 1 publications below |
RIP | See 1 publications below |
Product Information
11302-1-AP targets Ribosomal protein L4 in WB, IHC, IF/ICC, FC (Intra), IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rice, arabidopsis |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Ribosomal protein L4 fusion protein Ag1828 相同性解析による交差性が予測される生物種 |
Full Name | ribosomal protein L4 |
Calculated molecular weight | 48 kDa |
Observed molecular weight | 48 kDa |
GenBank accession number | BC009888 |
Gene symbol | Ribosomal protein L4 |
Gene ID (NCBI) | 6124 |
RRID | AB_2181909 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
60S ribosomal protein L4 is encoded by RPL4 (or RPL1) gene. RPL4 is a component of 60S subunit of ribosome, and belongs to the L4E family. By interacting with c-Myb, RPL4 plays a important role in c-myc expression in response to growth factor and nutritional signals. Recently RPL4 was reported to be able to increase effeiciency of viral recoding sequence.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for Ribosomal protein L4 antibody 11302-1-AP | Download protocol |
IHC protocol for Ribosomal protein L4 antibody 11302-1-AP | Download protocol |
IF protocol for Ribosomal protein L4 antibody 11302-1-AP | Download protocol |
IP protocol for Ribosomal protein L4 antibody 11302-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Cell Res Pre-ribosomal RNA reorganizes DNA damage repair factors in nucleus during meiotic prophase and DNA damage response. | ||
Mol Cell RAPIDASH: Tag-free enrichment of ribosome-associated proteins reveals composition dynamics in embryonic tissue, cancer cells, and macrophages | ||
Mol Cell N6-Methyladenosine Guides mRNA Alternative Translation during Integrated Stress Response. | ||
Mol Cell The G3BP1-Family-USP10 Deubiquitinase Complex Rescues Ubiquitinated 40S Subunits of Ribosomes Stalled in Translation from Lysosomal Degradation. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Anita (Verified Customer) (10-30-2020) | 1:1000 dilution works for HeLa cells, not for C. elegans nematodes
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