RPL5 Polyclonal antibody
RPL5 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human, Monkey and More (1)
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
Cat no : 29092-1-AP
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | COS-7 cells, MOLT-4 cells, PC-3 cells |
| Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 3 publications below |
| IHC | See 1 publications below |
Product Information
29092-1-AP targets RPL5 in WB, IHC, ELISA applications and shows reactivity with Human, Monkey samples.
| Tested Reactivity | Human, Monkey |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | RPL5 fusion protein Ag30837 相同性解析による交差性が予測される生物種 |
| Full Name | ribosomal protein L5 |
| Observed molecular weight | 34 kDa |
| GenBank accession number | BC109370 |
| Gene symbol | RPL5 |
| Gene ID (NCBI) | 6125 |
| RRID | AB_2881240 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
Background Information
The mammalian ribosome is a macromolecular assembly of 4 RNA species (rRNAs) and approximately 80 different proteins, including RPL5. RPL5 (ribosome protein L15) is rquired for rRNA maturation and formation of the 60S ribosomal subunits, and defects or mutation in RPL5 is the cause of DBA6.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RPL5 antibody 29092-1-AP | Download protocol |
| IHC protocol for RPL5 antibody 29092-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Rep mTOR- and LARP1-dependent regulation of TOP mRNA poly(A) tail and ribosome loading | ||
BMC Mol Cell Biol Ribosomal protein RPL5 regulates colon cancer cell proliferation and migration through MAPK/ERK signaling pathway
| ||
Front Oncol Spatially resolved transcriptomics revealed local invasion-related genes in colorectal cancer | ||
iScience Differential toxicity and localization of arginine-rich C9ORF72 dipeptide repeat proteins depend on de-clustering of positive charges |
