RPL7A Polyclonal antibody
RPL7A Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, IP, ELISA
Conjugate
Unconjugated
Cat no : 15340-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | MCF7 cells, MCF-7 cells, mouse kidney tissue, mouse liver tissue, rat kidney tissue |
| Positive IP detected in | mouse kidney tissue |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 5 publications below |
| IF | See 2 publications below |
| IP | See 1 publications below |
Product Information
15340-1-AP targets RPL7A in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | RPL7A fusion protein Ag7543 相同性解析による交差性が予測される生物種 |
| Full Name | ribosomal protein L7a |
| Calculated molecular weight | 266 aa, 30 kDa |
| Observed molecular weight | 30-32 kDa |
| GenBank accession number | BC005128 |
| Gene symbol | RPL7A |
| Gene ID (NCBI) | 6130 |
| RRID | AB_2254051 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Ribosomal proteins are a major component of ribosomes, which catalyze protein synthesis. RPL7a, which is a component of the 60S large ribosomal subunit, has additional functions involved in cell growth and differentiation that occur via interaction with human thyroid hormone receptor (THR) and retinoic acid receptor (RAR) and in turn inhibit the activities of the two nuclear hormone receptors [PMID:21505254].
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RPL7A antibody 15340-1-AP | Download protocol |
| IHC protocol for RPL7A antibody 15340-1-AP | Download protocol |
| IF protocol for RPL7A antibody 15340-1-AP | Download protocol |
| IP protocol for RPL7A antibody 15340-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Regnase-1 and Roquin Regulate a Common Element in Inflammatory mRNAs by Spatiotemporally Distinct Mechanisms. | ||
Mol Cell Estrogen-Related Hormones Induce Apoptosis by Stabilizing Schlafen-12 Protein Turnover. | ||
Acta Neuropathol Commun Inefficient quality control of ribosome stalling during APP synthesis generates CAT-tailed species that precipitate hallmarks of Alzheimer's disease | ||
Nucleic Acids Res The induction of p53 correlates with defects in the production, but not the levels, of the small ribosomal subunit and stalled large ribosomal subunit biogenesis | ||
Am J Cancer Res UBE2S targets RPL26 for ubiquitination and degradation to promote non-small cell lung cancer progression via regulating c-Myc | ||
Nat Commun Nuclear and cytoplasmic specific RNA binding proteome enrichment and its changes upon ferroptosis induction |
