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Species Reactivity

Species Reactivity

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  • Featured Product
  • KD/KO Validated

RPL8 Polyclonal antibody

RPL8 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 16981-1-AP

Synonyms

60S ribosomal protein L8, ribosomal protein L8, RPL8

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RPL8 Polyclonal antibody (16981-1-AP)

    WB analysis using 16981-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16981-1-AP (RPL8 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using RPL8 Polyclonal antibody (16981-1-AP)

    WB analysis of HepG2 using 16981-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 16981-1-AP (RPL8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inHepG2 cells, MCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16981-1-AP targets RPL8 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RPL8 fusion protein Ag9957 相同性解析による交差性が予測される生物種
Full Name ribosomal protein L8
Calculated molecular weight 257 aa, 28 kDa
Observed molecular weight 28-34 kDa
GenBank accession numberBC012197
Gene symbol RPL8
Gene ID (NCBI) 6132
RRIDAB_2182071
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RPL8 antibody 16981-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Mol Cell

RAPIDASH: Tag-free enrichment of ribosome-associated proteins reveals composition dynamics in embryonic tissue, cancer cells, and macrophages

Authors - Teodorus Theo Susanto
View Article
humanIF

Cell Discov

Ribosomal RNA regulates chromosome clustering during mitosis.

Authors - Kai Ma
View Article
humanWB

Genes (Basel)

Transcriptional Regulation of CD40 Expression by 4 Ribosomal Proteins via a Functional SNP on a Disease-Associated CD40 Locus.

Authors - Meijuan Zou
  • KD Validated
View Article