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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RPLP0 Polyclonal antibody

RPLP0 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 51019-2-AP

Synonyms

60S ribosomal protein L10E, L10E, P0, PRLP0, ribosomal protein, large, P0, RPLP0, RPP0

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using RPLP0 Polyclonal antibody (51019-2-AP)

    WB analysis of HEK-293 using 51019-2-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 51019-2-AP (RPLP0 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using RPLP0 Polyclonal antibody (51019-2-AP)

    IHC staining of human breast cancer using 51019-2-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 51019-2-AP (RPLP0 antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

Positive WB detected inHEK-293 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

51019-2-AP targets RPLP0 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name ribosomal protein, large, P0
Calculated molecular weight 34 kDa
Observed molecular weight 34 kDa
GenBank accession numberBC009867
Gene symbol RPLP0
Gene ID (NCBI) 6175
RRIDAB_2238697
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RPLP0 antibody 51019-2-APDownload protocol
IHC protocol for RPLP0 antibody 51019-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols