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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RPLP0 Monoclonal antibody (68056-1-Ig)

    WB analysis using 68056-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68056-1-Ig (RPLP0 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using RPLP0 Monoclonal antibody (68056-1-Ig)

    WB analysis using 68056-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68056-1-Ig (RPLP0 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using RPLP0 Monoclonal antibody (68056-1-Ig)

    IHC staining of human colon cancer using 68056-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 68056-1-Ig (RPLP0 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using RPLP0 Monoclonal antibody (68056-1-Ig)

    IHC staining of human colon cancer using 68056-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 68056-1-Ig (RPLP0 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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RPLP0 Monoclonal antibody

RPLP0 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 68056-1-Ig
Clone No.2A2E1
Publications(1)

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse , Rat and More (1)

Applications

WB, IHC, ELISA

Synonyms

60S ribosomal protein L10E, L10E, P0, PRLP0, ribosomal protein, large, P0, RPLP0, RPP0

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, U2OS cells, A549 cells, A375 cells, A431 cells, NCI-H1299 cells, PC-12 cells, NIH/3T3 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68056-1-Ig targets RPLP0 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse , Rat samples.

Tested Reactivity Human, Mouse , Rat
Cited Reactivity rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RPLP0 fusion protein Ag1829 相同性解析による交差性が予測される生物種
Full Name ribosomal protein, large, P0
Calculated molecular weight 34 kDa
Observed molecular weight34 kDa
GenBank accession numberBC009867
Gene Symbol RPLP0
Gene ID (NCBI) 6175
RRIDAB_2918797
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP05388
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RPLP0 antibody 68056-1-IgDownload protocol
IHC protocol for RPLP0 antibody 68056-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
rat

Biomolecules

Dietary Soy Prevents Alcohol-Mediated Neurocognitive Dysfunction and Associated Impairments in Brain Insulin Pathway Signaling in an Adolescent Rat Model.

Authors - Ming Tong
View Article