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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RPLP0 Monoclonal antibody

RPLP0 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse , Rat and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2A2E1

Cat no : 68056-1-Ig

Synonyms

60S ribosomal protein L10E, L10E, P0, PRLP0, ribosomal protein, large, P0, RPLP0, RPP0

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RPLP0 Monoclonal antibody (68056-1-Ig)

    WB analysis using 68056-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68056-1-Ig (RPLP0 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using RPLP0 Monoclonal antibody (68056-1-Ig)

    WB analysis using 68056-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68056-1-Ig (RPLP0 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using RPLP0 Monoclonal antibody (68056-1-Ig)

    IHC staining of human colon cancer using 68056-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 68056-1-Ig (RPLP0 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using RPLP0 Monoclonal antibody (68056-1-Ig)

    IHC staining of human colon cancer using 68056-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 68056-1-Ig (RPLP0 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHeLa cells, U2OS cells, A549 cells, A375 cells, A431 cells, NCI-H1299 cells, PC-12 cells, NIH/3T3 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68056-1-Ig targets RPLP0 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse , Rat samples.

Tested Reactivity Human, Mouse , Rat
Cited Reactivity rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RPLP0 fusion protein Ag1829 相同性解析による交差性が予測される生物種
Full Name ribosomal protein, large, P0
Calculated molecular weight 34 kDa
Observed molecular weight34 kDa
GenBank accession numberBC009867
Gene symbol RPLP0
Gene ID (NCBI) 6175
RRIDAB_2918797
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RPLP0 antibody 68056-1-IgDownload protocol
IHC protocol for RPLP0 antibody 68056-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
rat

Biomolecules

Dietary Soy Prevents Alcohol-Mediated Neurocognitive Dysfunction and Associated Impairments in Brain Insulin Pathway Signaling in an Adolescent Rat Model.

Authors - Ming Tong
View Article