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- KD/KO Validated
RPLP1 Polyclonal antibody
RPLP1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (1)
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
Cat no : 21636-1-AP
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells |
| Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 5 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
Product Information
21636-1-AP targets RPLP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | RPLP1 fusion protein Ag16268 相同性解析による交差性が予測される生物種 |
| Full Name | ribosomal protein, large, P1 |
| Calculated molecular weight | 12 kDa |
| Observed molecular weight | 15-20 kDa |
| GenBank accession number | BC003369 |
| Gene symbol | RPLP1 |
| Gene ID (NCBI) | 6176 |
| RRID | AB_2878894 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
60S acidic ribosomal protein P1 (RRP1) is a 114 amino acid protein, which belongs to the ribosomal protein L12P family. RRP1 plays an important role in the elongation step of protein synthesis. The molecular weight of modified RRP1 is 15-20 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RPLP1 antibody 21636-1-AP | Download protocol |
| IHC protocol for RPLP1 antibody 21636-1-AP | Download protocol |
| IF protocol for RPLP1 antibody 21636-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Virulence RPLP1, an NS4B-interacting protein, enhances production of CSFV through promoting translation of viral genome.
| ||
Mol Hum Reprod 60S acidic ribosomal protein P1 (RPLP1) is elevated in human endometriotic tissue and in a murine model of endometriosis and is essential for endometriotic epithelial cell survival in vitro. | ||
Int J Mol Sci RPLP1 Is Up-Regulated in Human Adenomyosis and Endometrial Adenocarcinoma Epithelial Cells and Is Essential for Cell Survival and Migration In Vitro | ||
Am J Cancer Res Unveiling the prognostic implications of RPLP1 upregulation in osteosarcoma | ||
