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KD/KO Validated

Ribophorin I Polyclonal antibody

Name: Ribophorin I Polyclonal antibody
Brand: Proteintech
SKU: 12894-1-AP

Ribophorin I Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 12894-1-AP

Synonyms

RPN1, RPN-I, Ribophorin-1, Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1, Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 67 kDa subunit

Validation Data Gallery

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AllWBIHC

WB analysis using 12894-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12894-1-AP (RPN1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 12894-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 12894-1-AP (RPN1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IHC staining of mouse lung using 12894-1-AP
Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 12894-1-AP (RPN1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse lung using 12894-1-AP
Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 12894-1-AP (RPN1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat liver using 12894-1-AP
Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 12894-1-AP (RPN1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat liver using 12894-1-AP
Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 12894-1-AP (RPN1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Cat No. 12894-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HT-29 cells, K-562 cells, HeLa cells
Positive IHC detected in	mouse lung tissue, rat liver tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:12000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 13 publications below
IHC	See 2 publications below

Product Information

12894-1-AP targets Ribophorin I in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, yeast
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Ribophorin I fusion protein Ag3576 相同性解析による交差性が予測される生物種
Full Name	ribophorin I
Calculated molecular weight	69 kDa
Observed molecular weight	66-69 kDa
GenBank accession number	BC010839
Gene symbol	RPN1
Gene ID (NCBI)	6184
RRID	AB_2170474
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for Ribophorin I antibody 12894-1-AP	Download protocol
IHC protocol for Ribophorin I antibody 12894-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
yeast	WB	Nature Reconfiguration of the proteasome during chaperone-mediated assembly. Authors - Soyeon Park View Article
mouse	WB	Nat Cell Biol Manganese regulation of COPII condensation controls circulating lipid homeostasis Authors - Xiao Wang View Article
human	WB	Sci Adv The translocon-associated protein (TRAP) complex regulates quality control of N-linked glycosylation during ER stress. Authors - Chatchai Phoomak View Article
human	WB	Nat Chem Biol Oligosaccharyltransferase inhibition induces senescence in RTK-driven tumor cells. Authors - Cecilia Lopez-Sambrooks View Article
rat	WB	Int J Mol Sci Impaired 26S Proteasome Assembly Precedes Neuronal Loss in Mutant UBQLN2 Rats. Authors - Wenjuan Zhang View Article
human	WB	Proteomics Changes in cellular glycosylation of leukemia cells upon treatment with acridone derivatives yield insight into drug action. Authors - Yini Wang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Ribophorin I Polyclonal antibody

Ribophorin I Polyclonal Antibody for WB, IHC, ELISA

Validation Data Gallery

WB analysis using 12894-1-AP

WB analysis of K-562 using 12894-1-AP

IHC staining of mouse lung using 12894-1-AP

IHC staining of mouse lung using 12894-1-AP

IHC staining of rat liver using 12894-1-AP

IHC staining of rat liver using 12894-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Reconfiguration of the proteasome during chaperone-mediated assembly.

Manganese regulation of COPII condensation controls circulating lipid homeostasis

The translocon-associated protein (TRAP) complex regulates quality control of N-linked glycosylation during ER stress.

Oligosaccharyltransferase inhibition induces senescence in RTK-driven tumor cells.

Impaired 26S Proteasome Assembly Precedes Neuronal Loss in Mutant UBQLN2 Rats.

Changes in cellular glycosylation of leukemia cells upon treatment with acridone derivatives yield insight into drug action.