Validation Data Gallery
Tested Applications
| Positive WB detected in | HT-29 cells, K-562 cells, HeLa cells |
| Positive IHC detected in | mouse lung tissue, rat liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 15 publications below |
| IHC | See 3 publications below |
Product Information
12894-1-AP targets Ribophorin I in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, yeast |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Ribophorin I fusion protein Ag3576 相同性解析による交差性が予測される生物種 |
| Full Name | ribophorin I |
| Calculated molecular weight | 69 kDa |
| Observed molecular weight | 66-69 kDa |
| GenBank accession number | BC010839 |
| Gene Symbol | RPN1 |
| Gene ID (NCBI) | 6184 |
| RRID | AB_2170474 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P04843 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Ribophorin I, also named as RPN1, is a key protein component of the oligosaccharyltransferase (OST) complex, which is embedded in the membrane of the rough endoplasmic reticulum (RER) (PMID: 38373565). It plays a crucial role in the process of N-linked glycosylation, a post-translational modification essential for the proper folding, stability, and function of many secretory and membrane-bound proteins (PMID: 17264154). Ribophorin I, along with Ribophorin II, forms a heterodimeric complex that facilitates the transfer of oligosaccharides from a lipid-linked donor to specific asparagine residues on nascent polypeptide chains (PMID: 30940778).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Ribophorin I antibody 12894-1-AP | Download protocol |
| IHC protocol for Ribophorin I antibody 12894-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Cell Biol Manganese regulation of COPII condensation controls circulating lipid homeostasis | ||
Sci Adv The translocon-associated protein (TRAP) complex regulates quality control of N-linked glycosylation during ER stress. | ||
Nat Chem Biol Oligosaccharyltransferase inhibition induces senescence in RTK-driven tumor cells. | ||
Int J Mol Sci Impaired 26S Proteasome Assembly Precedes Neuronal Loss in Mutant UBQLN2 Rats. | ||
Proteomics Changes in cellular glycosylation of leukemia cells upon treatment with acridone derivatives yield insight into drug action. |