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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Ribophorin I Recombinant antibody

Ribophorin I Recombinant Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240044D4

Cat no : 83250-2-RR

Synonyms

RPN1, RPN-I, Ribophorin-1, Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1, Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 67 kDa subunit

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RPN1 Recombinant antibody (83250-2-RR)

    WB analysis using 83250-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83250-2-RR (RPN1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using Ribophorin I Recombinant antibody (83250-2-RR)

    IHC staining of human stomach cancer using 83250-2-RR

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83250-2-RR (Ribophorin I antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer(pH9).

  • Flow cytometry (FC) experiment of HeLa cells using RPN1 Recombinant antibody (83250-2-RR)

    FC experiment of HeLa using 83250-2-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug RPN1 Recombinant antibody (83250-2-RR, Clone:240044D4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83250-2-RR

    Affinity and Kinetic Characterization of 83250-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83250-2-RR against Human RPN1 were performed. The affinity constant is 0.154 uM.

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Tested Applications

Positive WB detected inMCF-7 cells, HeLa cells, HepG2 cells, K-562 cells, mouse liver tissue, rat liver tissue
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83250-2-RR targets Ribophorin I in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Ribophorin I fusion protein Ag3576 相同性解析による交差性が予測される生物種
Full Name ribophorin I
Calculated molecular weight 69 kDa
Observed molecular weight 66-69 kDa
GenBank accession numberBC010839
Gene symbol RPN1
Gene ID (NCBI) 6184
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for Ribophorin I antibody 83250-2-RRDownload protocol
IHC protocol for Ribophorin I antibody 83250-2-RRDownload protocol
FC protocol for Ribophorin I antibody 83250-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols