- Featured Product
- KD/KO Validated
RPN2 Polyclonal antibody
RPN2 Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, RIP, IHC, ELISA
Conjugate
Unconjugated
Cat no : 10576-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | NIH/3T3 cells |
| Positive IP detected in | NIH/3T3 cells |
| Positive IHC detected in | human lung cancer tissue, human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
| IHC | See 2 publications below |
| RIP | See 1 publications below |
Product Information
10576-1-AP targets RPN2 in WB, IP, RIP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | RPN2 fusion protein Ag0862 相同性解析による交差性が予測される生物種 |
| Full Name | ribophorin II |
| Calculated molecular weight | 69 kDa |
| Observed molecular weight | 68-69 kDa |
| GenBank accession number | BC003560 |
| Gene symbol | RPN2 |
| Gene ID (NCBI) | 6185 |
| RRID | AB_2172805 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
RPN2 (Ribophorin II), an integral rough ER membrane glycoprotein, is part of an N-oligosaccharyl transferase complex and is involved in translocation and the maintenance of the structural uniqueness of the rough ER. RPN2 expression has been reported to be significantly associated with solid cancers, including breast cancer, esophageal squamous cell carcinoma, colorectal cancer, and lung squamous cell carcinoma.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RPN2 antibody 10576-1-AP | Download protocol |
| IHC protocol for RPN2 antibody 10576-1-AP | Download protocol |
| IP protocol for RPN2 antibody 10576-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Onco Targets Ther RPN2 Predicts Poor Prognosis and Promotes Bladder Cancer Growth and Metastasis via the PI3K-Akt Pathway. | ||
Onco Targets Ther Downregulation of ribophorin II suppresses tumor growth, migration, and invasion of nasopharyngeal carcinoma.
| ||
Res Sq Hexokinase-1 mitochondrial dissociation and protein O-GlcNAcylation drive heart failure with preserved ejection fraction | ||
Theranostics The N6-methyladenosine-mediated lncRNA WEE2-AS1 promotes glioblastoma progression by stabilizing RPN2 |
