• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RPP25 Polyclonal antibody

RPP25 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 15461-1-AP

Synonyms

RNase P protein subunit p25

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of HeLa cells using RPP25 Polyclonal antibody (15461-1-AP)

    WB analysis of HeLa using 15461-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15461-1-AP (RPP25 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using RPP25 Polyclonal antibody (15461-1-AP)

    WB analysis of MCF-7 using 15461-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 15461-1-AP (RPP25 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using RPP25 Polyclonal antibody (15461-1-AP)

    IP experiment of HeLa using 15461-1-AP

    IP result of anti-RPP25 (IP:15461-1-AP, 4ug; Detection:15461-1-AP 1:300) with HeLa cells lysate 2800 ug.

  • Immunohistochemistry (IHC) staining of human small intestine tissue using RPP25 Polyclonal antibody (15461-1-AP)

    IHC staining of human small intestine using 15461-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 15461-1-AP (RPP25 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using RPP25 Polyclonal antibody (15461-1-AP)

    IHC staining of human cervical cancer using 15461-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 15461-1-AP (RPP25 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human small intestine tissue using RPP25 Polyclonal antibody (15461-1-AP)

    IHC staining of human small intestine using 15461-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 15461-1-AP (RPP25 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using RPP25 Polyclonal antibody (15461-1-AP)

    IHC staining of human cervical cancer using 15461-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 15461-1-AP (RPP25 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RPP25 Polyclonal antibody (15461-1-AP)

    IF Staining of HeLa using 15461-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 15461-1-AP (RPP25 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

☆大好評トライアルサイズ ¥20,000～！ >>> 一次抗体特別価格キャンペーン開催中！

Tested Applications

Positive WB detected inHeLa cells, MCF-7 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman small intestine tissue, human cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

15461-1-AP targets RPP25 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RPP25 fusion protein Ag7742 相同性解析による交差性が予測される生物種
Full Name ribonuclease P/MRP 25kDa subunit
Calculated molecular weight 21 kDa
Observed molecular weight 25 kDa
GenBank accession numberBC002497
Gene symbol RPP25
Gene ID (NCBI) 54913
RRIDAB_2878142
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Ribonuclease P protein subunit p25(RPP25) is a component of ribonuclease P, which is a protein complex that generates mature tRNA molecular by cleaving their 5'-ends. It belongs to histone-like Alba family, also a component of RNase MRP. This subunit binds to RNA.

Protocols

Product Specific Protocols
WB protocol for RPP25 antibody 15461-1-APDownload protocol
IHC protocol for RPP25 antibody 15461-1-APDownload protocol
IF protocol for RPP25 antibody 15461-1-APDownload protocol
IP protocol for RPP25 antibody 15461-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Cell

Endoribonucleolytic Cleavage of m6A-Containing RNAs by RNase P/MRP Complex.

Authors - Ok Hyun Park
View Article