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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RPS11 Recombinant antibody (83517-3-RR)

    WB analysis using 83517-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83517-3-RR (RPS11 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using RPS11 Recombinant antibody (83517-3-RR)

    IHC staining of human stomach cancer using 83517-3-RR

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83517-3-RR (RPS11 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer(pH9).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using RPS11 Recombinant antibody (83517-3-RR)

    IHC staining of mouse testis using 83517-3-RR

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 83517-3-RR (RPS11 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using RPS11 Recombinant antibody (83517-3-RR)

    IHC staining of mouse testis using 83517-3-RR

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 83517-3-RR (RPS11 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using RPS11 Recombinant antibody (83517-3-RR)

    IF Staining of HepG2 using 83517-3-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RPS11 antibody (83517-3-RR, Clone: 240327D11 ) at dilution of 1:250 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of MCF-7 cells using RPS11 Recombinant antibody (83517-3-RR)

    FC experiment of MCF-7 using 83517-3-RR

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug RPS11 Recombinant antibody (83517-3-RR, Clone:240327D11) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83517-3-RR

    Affinity and Kinetic Characterization of 83517-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83517-3-RR against Human RPS11 were performed. The affinity constant is 5.05 nM.

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RPS11 Recombinant antibody

RPS11 Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 83517-3-RR
Clone No.240327D11

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Synonyms

Small ribosomal subunit protein uS17, ribosomal protein S11, 40S ribosomal protein S11, 240327D11

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration:  100 μL, 1000 ug/ml
20 μL, 1000 ug/ml
100 μL, 1000 ug/ml

¥85,000/ 100 μL

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Tested Applications

Positive WB detected inROS1728 cells, mouse testis tissue, rat testis tissue
Positive IHC detected inmouse testis tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83517-3-RR targets RPS11 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen RPS11 fusion protein Ag34848 相同性解析による交差性が予測される生物種
Full Name ribosomal protein S11
Calculated molecular weight 158 aa, 18 kDa
Observed molecular weight18 kDa
GenBank accession numberBC007945
Gene Symbol RPS11
Gene ID (NCBI) 6205
RRIDAB_3671142
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP62280
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RPS11 is a ribosomal protein involved in ribosome biogenesis. It is a component of the small ribosomal subunit. The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell.

Protocols

Product Specific Protocols
WB protocol for RPS11 antibody 83517-3-RRDownload protocol
IHC protocol for RPS11 antibody 83517-3-RRDownload protocol
IF protocol for RPS11 antibody 83517-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols