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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RPS12 Monoclonal antibody, PBS Only

RPS12 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A3D1

Cat no : 67683-1-PBS

Synonyms

40S ribosomal protein S12, ribosomal protein S12, RPS12

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RPS12 Monoclonal antibody (67683-1-Ig)

    WB analysis using 67683-1-Ig (same clone as 67683-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67683-1-Ig (RPS12 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67683-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NCI-H1299 cells using RPS12 Monoclonal antibody (67683-1-Ig)

    WB analysis of NCI-H1299 using 67683-1-Ig (same clone as 67683-1-PBS)

    NCI-H1299 cells were subjected to SDS PAGE followed by western blot with 67683-1-Ig (RPS12 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67683-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using RPS12 Monoclonal antibody (67683-1-Ig)

    IHC staining of human breast cancer using 67683-1-Ig (same clone as 67683-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67683-1-Ig (RPS12 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67683-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using RPS12 Monoclonal antibody (67683-1-Ig)

    IHC staining of human colon cancer using 67683-1-Ig (same clone as 67683-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67683-1-Ig (RPS12 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67683-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using RPS12 Monoclonal antibody (67683-1-Ig)

    IHC staining of human colon cancer using 67683-1-Ig (same clone as 67683-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67683-1-Ig (RPS12 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67683-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using RPS12 Monoclonal antibody (67683-1-Ig)

    IHC staining of human breast cancer using 67683-1-Ig (same clone as 67683-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67683-1-Ig (RPS12 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67683-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using RPS12 Monoclonal antibody (67683-1-Ig)

    IF Staining of HepG2 using 67683-1-Ig (same clone as 67683-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RPS12 antibody (67683-1-Ig, Clone: 1A3D1 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67683-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67683-1-PBS targets RPS12 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RPS12 fusion protein Ag9649 相同性解析による交差性が予測される生物種
Full Name ribosomal protein S12
Calculated molecular weight 132 aa, 15 kDa
Observed molecular weight15 kDa
GenBank accession numberBC017321
Gene symbol RPS12
Gene ID (NCBI) 6206
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

RPS12 (ribosomal protein S12) is a plastid ribosomal protein which is a part of the 30S ribosomal subunit. Together with S4 and S5 plays an important role in translational accuracy.