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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RPS14 Monoclonal antibody, PBS Only

RPS14 Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3D5H5

Cat no : 67566-1-PBS

Synonyms

40S ribosomal protein S14, EMTB, ribosomal protein S14, RPS14

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RPS14 Monoclonal antibody (67566-1-Ig)

    WB analysis using 67566-1-Ig (same clone as 67566-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67566-1-Ig (RPS14 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67566-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using RPS14 Monoclonal antibody (67566-1-Ig)

    WB analysis using 67566-1-Ig (same clone as 67566-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67566-1-Ig (RPS14 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67566-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using RPS14 Monoclonal antibody (67566-1-Ig)

    WB analysis using 67566-1-Ig (same clone as 67566-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67566-1-Ig (RPS14 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67566-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human urothelial carcinoma tissue using RPS14 Monoclonal antibody (67566-1-Ig)

    IHC staining of human urothelial carcinoma using 67566-1-Ig (same clone as 67566-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 67566-1-Ig (RPS14 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67566-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human urothelial carcinoma tissue using RPS14 Monoclonal antibody (67566-1-Ig)

    IHC staining of human urothelial carcinoma using 67566-1-Ig (same clone as 67566-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 67566-1-Ig (RPS14 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67566-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using RPS14 Monoclonal antibody (67566-1-Ig)

    IF Staining of HepG2 using 67566-1-Ig (same clone as 67566-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 67566-1-Ig (RPS14 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67566-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67566-1-PBS targets RPS14 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen RPS14 fusion protein Ag30032 相同性解析による交差性が予測される生物種
Full Name ribosomal protein S14
Calculated molecular weight 16 kDa
Observed molecular weight 16 kDa
GenBank accession numberBC003401
Gene symbol RPS14
Gene ID (NCBI) 6208
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

The mammalian ribosome consists of 4 RNA species and approximately 80 different proteins. The ribosomal proteins are encoded by complex gene families that include at least 1 active intron-containing gene and multiple processed pseudogenes[ PMID 3785212]. The RPS14 gene, encoding a ribosomal protein that belongs to the 40S ribosome, and mapping to the commonly deleted region (CDR) of the 5q- syndrome, shows haploinsufficiency in the CD34+ cells of patients with this disorder[PMID:22430631].