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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RPS15A Polyclonal antibody

RPS15A Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14942-1-AP

Synonyms

40S ribosomal protein S15a, ribosomal protein S15a, RPS15A, S15a

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RPS15A Polyclonal antibody (14942-1-AP)

    WB analysis using 14942-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14942-1-AP (RPS15A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using RPS15A Polyclonal antibody (14942-1-AP)

    IHC staining of human kidney using 14942-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14942-1-AP (RPS15A antibody) at dilution of 1:50 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using RPS15A Polyclonal antibody (14942-1-AP)

    IHC staining of human kidney using 14942-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14942-1-AP (RPS15A antibody) at dilution of 1:50 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHeLa cells, HepG2 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14942-1-AP targets RPS15A in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RPS15A fusion protein Ag6655 相同性解析による交差性が予測される生物種
Full Name ribosomal protein S15a
Calculated molecular weight 15 kDa
Observed molecular weight13-15 kDa
GenBank accession numberBC001697
Gene symbol RPS15A
Gene ID (NCBI) 6210
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RPS15A is a component of the small ribosomal subunit, it is required for proper erythropoiesis.

Protocols

Product Specific Protocols
WB protocol for RPS15A antibody 14942-1-APDownload protocol
IHC protocol for RPS15A antibody 14942-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Cancer Med

MCM8 promotes gastric cancer progression through RPS15A and predicts poor prognosis

Authors - Lixian Ding
View Article