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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RPS16 Polyclonal antibody

RPS16 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 15603-1-AP

Synonyms

Small ribosomal subunit protein uS9, ribosomal protein S16, 40S ribosomal protein S16

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using RPS16 Polyclonal antibody (15603-1-AP)

    WB analysis of HeLa using 15603-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15603-1-AP (RPS16 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using RPS16 Polyclonal antibody (15603-1-AP)

    WB analysis of Jurkat using 15603-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 15603-1-AP (RPS16 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using RPS16 Polyclonal antibody (15603-1-AP)

    IHC staining of human breast cancer using 15603-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 15603-1-AP (RPS16 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using RPS16 Polyclonal antibody (15603-1-AP)

    IHC staining of human breast cancer using 15603-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 15603-1-AP (RPS16 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using RPS16 Polyclonal antibody (15603-1-AP)

    IF Staining of MCF-7 using 15603-1-AP

    Immunofluorescent analysis of MCF-7 cells, using RPS16 antibody 15603-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

Positive WB detected inHeLa cells, Jurkat cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15603-1-AP targets RPS16 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RPS16 fusion protein Ag7986 相同性解析による交差性が予測される生物種
Full Name ribosomal protein S16
Calculated molecular weight 16 kDa
Observed molecular weight 16 kDa
GenBank accession numberBC004324
Gene symbol RPS16
Gene ID (NCBI) 6217
RRIDAB_2180168
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RPS16, the 40S ribosmal protein S16, belongs to the ribosomal protein S9P family. It is located in the cytoplasm and can be acetylated. RPS16 is extensively involved in rRNA processing, translational elongation, initiation and termination via its RNA binding activity. Catalog#15603-1-AP is a rabbit polyclonal antibody raised against full-length RPS16 of human origin.

Protocols

Product Specific Protocols
WB protocol for RPS16 antibody 15603-1-APDownload protocol
IHC protocol for RPS16 antibody 15603-1-APDownload protocol
IF protocol for RPS16 antibody 15603-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Proteomics Clin Appl

New insights into the functions and localization of nuclear CCT protein complex in K562 leukemia cells.

Authors - Huang Rui R
View Article
humanIHC

Int J Biol Markers

Potential prognostic biomarkers of hepatocellular carcinoma based on 4D label-free quantitative proteomics analysis pilot investigation

Authors - Lida Suo
View Article