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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RPS17 Recombinant antibody

RPS17 Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240851H3

Cat no : 83848-4-RR

Synonyms

RPS17L1, RPS17L, ribosomal protein S17, DBA4, 40S ribosomal protein S17

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RPS17 Recombinant antibody (83848-4-RR)

    WB analysis using 83848-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83848-4-RR (RPS17 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using RPS17 Recombinant antibody (83848-4-RR)

    IF Staining of U2OS using 83848-4-RR

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using RPS17 antibody (83848-4-RR, Clone: 240851H3 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Flow cytometry (FC) experiment of HeLa cells using RPS17 Recombinant antibody (83848-4-RR)

    FC experiment of HeLa using 83848-4-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug RPS17 Recombinant antibody (83848-4-RR, Clone:240851H3) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83848-4-RR

    Affinity and Kinetic Characterization of 83848-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83848-4-RR against Human RPS17 were performed. The affinity constant is 21.2 pM.

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Tested Applications

Positive WB detected inHeLa cells, A549 cells, Jurkat cells, HEK-293 cells
Positive IF/ICC detected inU2OS cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83848-4-RR is RPS17 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen RPS17 fusion protein Ag9334 相同性解析による交差性が予測される生物種
Full Name ribosomal protein S17
Calculated molecular weight 135 aa, 16 kDa
Observed molecular weight16 kDa
GenBank accession numberBC009407
Gene symbol RPS17
Gene ID (NCBI) 6218
RRIDAB_3671434
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RPS17 is a component of the small ribosomal subunit, the ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell.

Protocols

Product Specific Protocols
WB protocol for RPS17 antibody 83848-4-RRDownload protocol
IF protocol for RPS17 antibody 83848-4-RRDownload protocol
FC protocol for RPS17 antibody 83848-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols