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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RPS18 Recombinant antibody

RPS18 Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240004C10

Cat no : 83327-1-RR

Synonyms

Ke-3, KE3, KE 3, HKE3, D6S218E

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using RPS18 Recombinant antibody (83327-1-RR)

    IF Staining of HepG2 using 83327-1-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RPS18 antibody (83327-1-RR, Clone: 240004C10 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Flow cytometry (FC) experiment of HepG2 cells using RPS18 Recombinant antibody (83327-1-RR)

    FC experiment of HepG2 using 83327-1-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug RPS18 Recombinant antibody (83327-1-RR, Clone:240004C10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83327-1-RR

    Affinity and Kinetic Characterization of 83327-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83327-1-RR against Human RPS18 were performed. The affinity constant is 0.47 nM.

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Tested Applications

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83327-1-RR is RPS18 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen RPS18 fusion protein Ag12505 相同性解析による交差性が予測される生物種
Full Name ribosomal protein S18
Calculated molecular weight 152 aa, 18 kDa
GenBank accession numberBC106063
Gene symbol RPS18
Gene ID (NCBI) 6222
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for RPS18 antibody 83327-1-RRDownload protocol
FC protocol for RPS18 antibody 83327-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols