RPS19 Polyclonal antibody

Name: RPS19 Polyclonal antibody
Brand: Proteintech
SKU: 15085-1-AP
Rating: 5.00 (1 reviews)

RPS19 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

Reviews (1)

Cat no : 15085-1-AP

Synonyms

40S ribosomal protein S19, DBA, ribosomal protein S19, RPS19

Validation Data Gallery

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AllWBIPIHC

WB analysis using 15085-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15085-1-AP (RPS19 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of human testis using 15085-1-AP
human testis tissue were subjected to SDS PAGE followed by western blot with 15085-1-AP (RPS19 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of human testis using 15085-1-AP
human testis tissue were subjected to SDS PAGE followed by western blot with 15085-1-AP (RPS19 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of human testis using 15085-1-AP
human testis tissue were subjected to SDS PAGE followed by western blot with 15085-1-AP (RPS19 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 15085-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 15085-1-AP (RPS19 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 15085-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 15085-1-AP (RPS19 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of human testis using 15085-1-AP
human testis tissue were subjected to SDS PAGE followed by western blot with 15085-1-AP (RPS19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of rat colon using 15085-1-AP
rat colon tissue were subjected to SDS PAGE followed by western blot with 15085-1-AP (RPS19 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 15085-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 15085-1-AP (RPS19 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of mouse testis using 15085-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 15085-1-AP (RPS19 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of mouse testis using 15085-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 15085-1-AP (RPS19 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IP experiment of HEK-293 using 15085-1-AP
IP result of anti-RPS19 (IP:15085-1-AP, 4ug; Detection:15085-1-AP 1:500) with HEK-293 cells lysate 880ug.
IHC staining of human colon cancer using 15085-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer using 15085-1-AP (RPS19 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human colon cancer using 15085-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer using 15085-1-AP (RPS19 antibody) at dilution of 1:100 (under 40x lens).

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Cat No. 15085-1-AP

Size/Concentration

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	Caco-2 cells, human testis tissue, HEK-293 cells, rat colon tissue, mouse testis tissue, HeLa cells, K-562 cells
Positive IP detected in	HEK-293 cells
Positive IHC detected in	human colon cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:3000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 8 publications below
IHC	See 1 publications below
IF	See 1 publications below

Product Information

15085-1-AP targets RPS19 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	RPS19 fusion protein Ag7164 相同性解析による交差性が予測される生物種
Full Name	ribosomal protein S19
Calculated molecular weight	16 kDa
Observed molecular weight	16 kDa
GenBank accession number	BC000023
Gene symbol	RPS19
Gene ID (NCBI)	6223
RRID	AB_2180202
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

The mammalian ribosome consists of 4 RNA species and approximately 80 different proteins. The ribosomal proteins are encoded by complex gene families that include at least 1 active intron-containing gene and multiple processed pseudogenes. The RPS19 protein is a component of the 40S ribosomal subunit [PMID: 17726054]. Diamond-Blackfan anemia (DBA) is a congenital erythroid hypoplasia caused by a functional haploinsufficiency of genes encoding for ribosomal proteins. Among these genes, ribosomal protein S19 (RPS19) is mutated most frequently [PMID:21989989]. It also a p53-related ribosomal protein that possibly involves cellular apoptosis through the BAX/p53 pathway [PMID:22272377].

Protocols

Product Specific Protocols
WB protocol for RPS19 antibody 15085-1-AP	Download protocol
IHC protocol for RPS19 antibody 15085-1-AP	Download protocol
IP protocol for RPS19 antibody 15085-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Nat Commun Kaposi's sarcoma-associated herpesvirus induces specialised ribosomes to efficiently translate viral lytic mRNAs Authors - James C Murphy View Article
	WB,IF	Development Msl3 promotes germline stem cell differentiation in female Drosophila. Authors - Alicia McCarthy View Article
mouse	IHC	J Proteome Res Human SOD1-G93A specific distribution evidenced in murine brain of a transgenic model for Amyotrophic Lateral Sclerosis by MALDI Imaging Mass Spectrometry. Authors - Elena Acquadro View Article
human	WB	Sci Rep RNA G-quadruplex structures control ribosomal protein production Authors - DhavalVarshney View Article
human	WB	BBA Clin Mutation of EMG1 causing Bowen-Conradi syndrome results in reduced cell proliferation rates concomitant with G2/M arrest and 18S rRNA processing delay. Authors - Joy Armistead View Article
mouse	WB	Adv Sci (Weinh) Fine-Tuning of Cholesterol Homeostasis Controls Erythroid Differentiation. Authors - Zhiyuan Lu View Article

Reviews

Add a review

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

H (Verified Customer) (04-06-2020)

RPS19 is an abundant protein. I could easily detect a clear band.

Applications: Western Blot
Primary Antibody Dilution: 1:1000
Cell Tissue Type: HEK293T

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RPS19 Polyclonal antibody

RPS19 Polyclonal Antibody for WB, IP, IHC, ELISA

Validation Data Gallery

WB analysis using 15085-1-AP

WB analysis of human testis using 15085-1-AP

WB analysis of human testis using 15085-1-AP

WB analysis of human testis using 15085-1-AP

WB analysis of HEK-293 using 15085-1-AP

WB analysis of HEK-293 using 15085-1-AP

WB analysis of human testis using 15085-1-AP

WB analysis of rat colon using 15085-1-AP

WB analysis of HEK-293 using 15085-1-AP

WB analysis of mouse testis using 15085-1-AP

WB analysis of mouse testis using 15085-1-AP

IP experiment of HEK-293 using 15085-1-AP

IHC staining of human colon cancer using 15085-1-AP

IHC staining of human colon cancer using 15085-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Kaposi's sarcoma-associated herpesvirus induces specialised ribosomes to efficiently translate viral lytic mRNAs

Msl3 promotes germline stem cell differentiation in female Drosophila.

Human SOD1-G93A specific distribution evidenced in murine brain of a transgenic model for Amyotrophic Lateral Sclerosis by MALDI Imaging Mass Spectrometry.

RNA G-quadruplex structures control ribosomal protein production

Mutation of EMG1 causing Bowen-Conradi syndrome results in reduced cell proliferation rates concomitant with G2/M arrest and 18S rRNA processing delay.

Fine-Tuning of Cholesterol Homeostasis Controls Erythroid Differentiation.

Reviews