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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RPS27L Polyclonal antibody

RPS27L Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 15871-1-AP

Synonyms

Small ribosomal subunit protein eS27-like, RPS, Ribosomal protein eS27-like, 40S ribosomal protein S27-like

Validation Data Gallery

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  • Western Blot (WB) analysis of SH-SY5Y cells using RPS27L Polyclonal antibody (15871-1-AP)

    WB analysis of SH-SY5Y using 15871-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 15871-1-AP (RPS27L antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using RPS27L Polyclonal antibody (15871-1-AP)

    WB analysis of HepG2 using 15871-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 15871-1-AP (RPS27L antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human heart tissue using RPS27L Polyclonal antibody (15871-1-AP)

    WB analysis of human heart using 15871-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 15871-1-AP (RPS27L antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using RPS27L Polyclonal antibody (15871-1-AP)

    WB analysis of mouse brain using 15871-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 15871-1-AP (RPS27L antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of SH-SY5Y cells using RPS27L Polyclonal antibody (15871-1-AP)

    IP experiment of SH-SY5Y using 15871-1-AP

    IP result of anti-RPS27L (IP:15871-1-AP, 4ug; Detection:15871-1-AP 1:300) with SH-SY5Y cells lysate 2160 ug.

  • Immunohistochemistry (IHC) staining of human stomach tissue using RPS27L Polyclonal antibody (15871-1-AP)

    IHC staining of human stomach using 15871-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 15871-1-AP (RPS27L antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using RPS27L Polyclonal antibody (15871-1-AP)

    IF Staining of SH-SY5Y using 15871-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed SH-SY5Y cells using 15871-1-AP (RPS27L antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inSH-SY5Y cells, mouse brain tissue, human heart tissue, HepG2 cells
Positive IP detected inSH-SY5Y cells
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

15871-1-AP targets RPS27L in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RPS27L fusion protein Ag8628 相同性解析による交差性が予測される生物種
Full Name ribosomal protein S27-like
Calculated molecular weight 84 aa, 10 kDa
Observed molecular weight 10 kDa
GenBank accession numberBC003667
Gene symbol RPS27L
Gene ID (NCBI) 51065
RRIDAB_2253903
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RPS27L antibody 15871-1-APDownload protocol
IHC protocol for RPS27L antibody 15871-1-APDownload protocol
IF protocol for RPS27L antibody 15871-1-APDownload protocol
IP protocol for RPS27L antibody 15871-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Elife

Subfunctionalized expression drives evolutionary retention of ribosomal protein paralogs Rps27 and Rps27l in vertebrates

Authors - Adele Francis Xu
View Article
humanWB

J Mol Biol

Defective Human SRP Induces Protein Quality Control and Triggers Stress Response

Authors - Elena B Tikhonova
View Article