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  • KD/KO Validated

RPS3 Polyclonal antibody

RPS3 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Cat no : 11990-1-AP

Synonyms

40S ribosomal protein S3, Cell and organelle markers, ribosomal protein S3, Ribosome Marker, RPS3


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Tested Applications

Positive WB detected inHEK-293 cells, mouse testis tissue, PC-12 cells, HeLa cells, MCF-7 cells, NIH/3T3 cells
Positive IP detected inmouse testis tissue
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11990-1-AP targets RPS3 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RPS3 fusion protein Ag3918 相同性解析による交差性が予測される生物種
Full Name ribosomal protein S3
Calculated molecular weight 26 aa, 7 kDa
Observed molecular weight 26.7 kDa
GenBank accession numberBC034149
Gene symbol RPS3
Gene ID (NCBI) 6188
RRIDAB_2180758
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

40S ribosomal protein S3 (RPS3), also named as SW-cl.26, is a 243 amino acid protein,which contains one KH type-2 domain and belongs to the ribosomal protein S3P family. RPS3 localizes in the cytoplasm. RPS3 is identified in a IGF2BP1-dependent mRNP granule complex, which contains untranslated mRNAs. RPS3 plays a role in repairing various DNA damage acting as a repair UV endonuclease. Nuclear accumulation of RPS3 results in an increase in DNA repair activity to some extent, thereby sustaining neuronal survival.

Protocols

Product Specific Protocols
WB protocol for RPS3 antibody 11990-1-APDownload protocol
IHC protocol for RPS3 antibody 11990-1-APDownload protocol
IP protocol for RPS3 antibody 11990-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

Kaposi's sarcoma-associated herpesvirus induces specialised ribosomes to efficiently translate viral lytic mRNAs

Authors - James C Murphy
mouseIF

Nat Commun

Cilia locally synthesize proteins to sustain their ultrastructure and functions.

Authors - Kai Hao
humanWB

Curr Biol

RPL10L Is Required for Male Meiotic Division by Compensating for RPL10 during Meiotic Sex Chromosome Inactivation in Mice.

Authors - Long Jiang
pigIF

J Anim Sci Biotechnol

Glyphosate exposure deteriorates oocyte meiotic maturation via induction of organelle dysfunctions in pigs.

Authors - Chunhua Xing
humanWB,IHC

Front Oncol

RPS3 Promotes the Metastasis and Cisplatin Resistance of Adenoid Cystic Carcinoma.

Authors - Xi Rui
humanIF

Front Immunol

Interactome of the Autoimmune Risk Protein ANKRD55.

Authors - Nerea Ugidos

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Boyan (Verified Customer) (12-16-2021)

This antibody recognized a clean band at 27 kd.

  • Applications: Western Blot
FH

Huanzhou (Verified Customer) (11-26-2018)

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Cl-16