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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RPS3 Polyclonal antibody

RPS3 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Cat no : 15198-1-AP

Synonyms

ribosomal protein S3, OK/SW-cl.26, FLJ27450, FLJ26283, EC:4.2.99.18

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using RPS3 Polyclonal antibody (15198-1-AP)

    WB analysis of mouse brain using 15198-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 15198-1-AP (RPS3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using RPS3 Polyclonal antibody (15198-1-AP)

    WB analysis of mouse brain using 15198-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 15198-1-AP (RPS3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat brain tissue using RPS3 Polyclonal antibody (15198-1-AP)

    WB analysis of rat brain using 15198-1-AP

    rat brain tissue were subjected to SDS PAGE followed by western blot with 15198-1-AP (RPS3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using RPS3 Polyclonal antibody (15198-1-AP)

    WB analysis of COLO 320 using 15198-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 15198-1-AP (RPS3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using RPS3 Polyclonal antibody (15198-1-AP)

    WB analysis of NIH/3T3 using 15198-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 15198-1-AP (RPS3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse colon tissue using RPS3 Polyclonal antibody (15198-1-AP)

    WB analysis of mouse colon using 15198-1-AP

    mouse colon tissue were subjected to SDS PAGE followed by western blot with 15198-1-AP (RPS3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using RPS3 Polyclonal antibody (15198-1-AP)

    WB analysis of HeLa using 15198-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15198-1-AP (RPS3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using RPS3 Polyclonal antibody (15198-1-AP)

    IP experiment of mouse brain using 15198-1-AP

    IP result of anti-RPS3 (IP:15198-1-AP, 4ug; Detection:15198-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using RPS3 Polyclonal antibody (15198-1-AP)

    IHC staining of human colon cancer using 15198-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15198-1-AP (RPS3 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using RPS3 Polyclonal antibody (15198-1-AP)

    IHC staining of human colon cancer using 15198-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15198-1-AP (RPS3 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using RPS3 Polyclonal antibody (15198-1-AP)

    IHC staining of human colon cancer using 15198-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15198-1-AP (RPS3 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using RPS3 Polyclonal antibody (15198-1-AP)

    IHC staining of human colon cancer using 15198-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15198-1-AP (RPS3 Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

Positive WB detected inmouse brain tissue, NIH/3T3 cells, mouse colon tissue, COLO 320 cells, HeLa cells, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

15198-1-AP targets RPS3 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityyeast
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RPS3 fusion protein Ag7346 相同性解析による交差性が予測される生物種
Full Name ribosomal protein S3
Calculated molecular weight 27 kDa
Observed molecular weight 33 kDa
GenBank accession numberBC003137
Gene symbol RPS3
Gene ID (NCBI) 6188
RRIDAB_2253905
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The ribosomal protein S3 is a subunit of 40S ribosomal protein, and has two distinct function. RPS3 acts as a ribosome protein that contributes to the domain of the ribosome where translation is initiated, and involves in repair of UV damage as an endonuclease. Besides binding RNA within the 40S ribosomal subunit, RPS3 participates in transcription, and apoptosis.

Protocols

Product Specific Protocols
WB protocol for RPS3 antibody 15198-1-APDownload protocol
IHC protocol for RPS3 antibody 15198-1-APDownload protocol
IP protocol for RPS3 antibody 15198-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
yeastWB

PLoS Genet

Role of Mex67-Mtr2 in the nuclear export of 40S pre-ribosomes.

Authors - Faza Marius B MB
View Article