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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RPS3 Recombinant antibody

RPS3 Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

242504F3

Cat no : 84947-4-RR

Synonyms

OK/SW-cl.26, FLJ27450, FLJ26283, EC:4.2.99.18, 40S ribosomal protein S3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RPS3 Recombinant antibody (84947-4-RR)

    WB analysis using 84947-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84947-4-RR (RPS3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84947-4-RR

    Affinity and Kinetic Characterization of 84947-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84947-4-RR against Human RPS3 were performed. The affinity constant is below 1 pM.

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Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, COLO 320 cells, NIH/3T3 cells, mouse brain tissue, human brain tissue,

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84947-4-RR targets RPS3 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen RPS3 fusion protein Ag7346 相同性解析による交差性が予測される生物種
Full Name ribosomal protein S3
Calculated molecular weight 27 kDa
Observed molecular weight 30 kDa
GenBank accession numberBC003137
Gene symbol RPS3
Gene ID (NCBI) 6188
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

40S ribosomal protein S3 (RPS3), also named as SW-cl.26, is a 243 amino acid protein,which contains one KH type-2 domain and belongs to the ribosomal protein S3P family. RPS3 localizes in the cytoplasm. RPS3 is identified in a IGF2BP1-dependent mRNP granule complex, which contains untranslated mRNAs. RPS3 plays a role in repairing various DNA damage acting as a repair UV endonuclease. Nuclear accumulation of RPS3 results in an increase in DNA repair activity to some extent, thereby sustaining neuronal survival.

Protocols

Product Specific Protocols
WB protocol for RPS3 antibody 84947-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols