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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RPS3A Polyclonal antibody (14123-1-AP)

    WB analysis using 14123-1-AP (same clone as 14123-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14123-1-AP (RPS3A antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14123-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A431 cells using RPS3A Polyclonal antibody (14123-1-AP)

    WB analysis of A431 using 14123-1-AP (same clone as 14123-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 14123-1-AP (RPS3A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14123-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using RPS3A Polyclonal antibody (14123-1-AP)

    WB analysis of Jurkat using 14123-1-AP (same clone as 14123-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 14123-1-AP (RPS3A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14123-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using RPS3A Polyclonal antibody (14123-1-AP)

    WB analysis of A549 using 14123-1-AP (same clone as 14123-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 14123-1-AP (RPS3A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14123-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using RPS3A Polyclonal antibody (14123-1-AP)

    IP experiment of HeLa using 14123-1-AP (same clone as 14123-1-PBS)

    IP result of anti-RPS3A (IP:14123-1-AP, 4ug; Detection:14123-1-AP 1:6000) with HeLa cells lysate 1600 ug. This data was developed using the same antibody clone with 14123-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using RPS3A Polyclonal antibody (14123-1-AP)

    IHC staining of human colon using 14123-1-AP (same clone as 14123-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14123-1-AP (RPS3A antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14123-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using RPS3A Polyclonal antibody (14123-1-AP)

    IF Staining of HepG2 using 14123-1-AP (same clone as 14123-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RPS3A antibody (14123-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14123-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using RPS3A Polyclonal antibody (14123-1-AP)

    FC experiment of HepG2 using 14123-1-AP (same clone as 14123-1-PBS)

    1X10^6 HepG2 cells were intracellularly stained with 0.5 ug Anti-Human RPS3A (14123-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 14123-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

RPS3A Polyclonal antibody, PBS Only

RPS3A Polyclonal Antibody for WB, IHC, FC (Intra), IP, Indirect ELISA
Cat No. 14123-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14123-1-PBS targets RPS3A in WB, IHC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RPS3A fusion protein Ag5273 相同性解析による交差性が予測される生物種
Full Name ribosomal protein S3A
Calculated molecular weight 30 kDa
Observed molecular weight 35 kDa
GenBank accession numberBC066926
Gene Symbol RPS3A
Gene ID (NCBI) 6189
RRIDAB_2253921
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP61247
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

RPS3a, a component of the ribosomal small subunit (40S), is a member of the A20 family of the deubiquitinating cysteine proteases. RPS3a is an inducing factor for oncogenic cellular transformation of Rat-1 cells by v-fos, and is highly expressed in most tumors including hepatocellular carcinoma and other cancers. On the other hand, RPS3a has also been described as a regulator of apoptosis and transcription factors