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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RPS4Y1 Polyclonal antibody

RPS4Y1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 17296-1-AP

Synonyms

Small ribosomal subunit protein eS4, Y isoform 1, RPS4Y, PRO2646, 40S ribosomal protein S4

Validation Data Gallery

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  • Western Blot (WB) analysis of DU 145 cells using RPS4Y1 Polyclonal antibody (17296-1-AP)

    WB analysis of DU 145 using 17296-1-AP

    DU 145 cells were subjected to SDS PAGE followed by western blot with 17296-1-AP (RPS4Y1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A2780 cells using RPS4Y1 Polyclonal antibody (17296-1-AP)

    WB analysis of A2780 using 17296-1-AP

    A2780 cells were subjected to SDS PAGE followed by western blot with 17296-1-AP (RPS4Y1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SKOV-3 cells using RPS4Y1 Polyclonal antibody (17296-1-AP)

    WB analysis of SKOV-3 using 17296-1-AP

    SKOV-3 cells were subjected to SDS PAGE followed by western blot with 17296-1-AP (RPS4Y1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using RPS4Y1 Polyclonal antibody (17296-1-AP)

    WB analysis of mouse testis using 17296-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 17296-1-AP (RPS4Y1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of DU 145 cells using RPS4Y1 Polyclonal antibody (17296-1-AP)

    WB analysis of DU 145 using 17296-1-AP

    DU 145 cells were subjected to SDS PAGE followed by western blot with 17296-1-AP (RPS4Y1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A2780 cells using RPS4Y1 Polyclonal antibody (17296-1-AP)

    WB analysis of A2780 using 17296-1-AP

    A2780 cells were subjected to SDS PAGE followed by western blot with 17296-1-AP (RPS4Y1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse ovary tissue using RPS4Y1 Polyclonal antibody (17296-1-AP)

    WB analysis of mouse ovary using 17296-1-AP

    mouse ovary tissue were subjected to SDS PAGE followed by western blot with 17296-1-AP (RPS4Y1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using RPS4Y1 Polyclonal antibody (17296-1-AP)

    WB analysis of HeLa using 17296-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 17296-1-AP (RPS4Y1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human appendicitis tissue using RPS4Y1 Polyclonal antibody (17296-1-AP)

    IHC staining of human appendicitis using 17296-1-AP

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 17296-1-AP (RPS4Y1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using RPS4Y1 Polyclonal antibody (17296-1-AP)

    IF Staining of HepG2 using 17296-1-AP

    Immunofluorescent analysis of HepG2 cells, using RPS4Y1 antibody 17296-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

Positive WB detected inDU 145 cells, A2780 cells, HeLa cells, mouse ovary tissue, mouse testis tissue, SKOV-3 cells
Positive IHC detected inhuman appendicitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

17296-1-AP targets RPS4Y1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RPS4Y1 fusion protein Ag11176 相同性解析による交差性が予測される生物種
Full Name ribosomal protein S4, Y-linked 1
Calculated molecular weight 263 aa, 29 kDa
Observed molecular weight 29 kDa
GenBank accession numberBC010286
Gene symbol RPS4Y1
Gene ID (NCBI) 6192
RRIDAB_2180889
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RPS4Y1, a Y-linked gene in the human, encodes a subunit of ribosomal protein S4, a component of the 40S subunit and 60S subunit. Ribosomal protein S4 is the only ribosomal protein known to be encoded by more than one gene, namely this gene, RPS4Y2 and the ribosomal protein S4, X-linked (RPS4X). RPS4Y maps to a 90-kb segment that has been implicated in Turner syndrome. As a homolog with RPS4X, the antibody can recognize endogenous level of RPS4Y and RPS4X, at 29-32kDa.

Protocols

Product Specific Protocols
WB protocol for RPS4Y1 antibody 17296-1-APDownload protocol
IHC protocol for RPS4Y1 antibody 17296-1-APDownload protocol
IF protocol for RPS4Y1 antibody 17296-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

iScience

Secretion of WNT7A by UC-MSCs assist in promoting the endometrial epithelial regeneration

Authors - Fangbo Liu
View Article