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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RPS6KA1 Polyclonal antibody (29224-1-AP)

    WB analysis using 29224-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29224-1-AP (RPS6KA1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using RPS6KA1 Polyclonal antibody (29224-1-AP)

    WB analysis of HeLa using 29224-1-AP

    WB result of RPS6KA1 antibody (29224-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RPS6KA1 transfected HeLa cells.

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  • KD/KO Validated

RPS6KA1 Polyclonal antibody

RPS6KA1 Polyclonal Antibody for WB, ELISA
Cat No. 29224-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

MAPKAPK 1a, MAPKAP kinase 1a, MAPK-activated protein kinase 1a, MAP kinase-activated protein kinase 1a, HU 1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inK-562 cells, HeLa cells, A431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29224-1-AP targets RPS6KA1 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name ribosomal protein S6 kinase, 90kDa, polypeptide 1
Calculated molecular weight 735 aa, 83 kDa
Observed molecular weight75-95 kDa
GenBank accession numberBC014966
Gene Symbol RPS6KA1
Gene ID (NCBI) 6195
RRIDAB_3669677
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ15418
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The 90 kDa ribosomal S6 kinases (RSK1-4) are a family of widely expressed serine/threonine kinases and play an important role in the MAPK signaling cascade and act downstream of ERK1/2 signaling. It can mediate mitogenic and stress-induced activation of the transcription factors CREB1, ETV1/ER81 and NR4A1/NUR77. RSK1 (RPS6KA1) is also designated as p90RSK associated with cellular proliferation, survival and differentiation.

Protocols

Product Specific Protocols
WB protocol for RPS6KA1 antibody 29224-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols