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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

S6K2 Polyclonal antibody

S6K2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 15268-1-AP

Synonyms

p70 S6 kinase beta, RPS6KB2, p70 ribosomal S6 kinase beta, P70 beta 2, P70 beta 1

Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using S6K2 Polyclonal antibody (15268-1-AP)

    WB analysis of MCF-7 using 15268-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 15268-1-AP (S6K2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using S6K2 Polyclonal antibody (15268-1-AP)

    WB analysis of MCF-7 using 15268-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 15268-1-AP (S6K2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of MCF-7 cells using S6K2 Polyclonal antibody (15268-1-AP)

    IP experiment of MCF-7 using 15268-1-AP

    IP result of anti-S6K2 (IP:15268-1-AP, 4ug; Detection:15268-1-AP 1:1000) with MCF-7 cells lysate 2800ug.

  • Immunohistochemistry (IHC) staining of human kidney tissue using S6K2 Polyclonal antibody (15268-1-AP)

    IHC staining of human kidney using 15268-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15268-1-AP (S6K2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using S6K2 Polyclonal antibody (15268-1-AP)

    IHC staining of human kidney using 15268-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15268-1-AP (S6K2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using S6K2 Polyclonal antibody (15268-1-AP)

    IF Staining of MCF-7 using 15268-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed MCF-7 cells using 15268-1-AP (S6K2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inMCF7 cells, MCF-7 cells
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15268-1-AP targets S6K2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen S6K2 fusion protein Ag7371 相同性解析による交差性が予測される生物種
Full Name ribosomal protein S6 kinase, 70kDa, polypeptide 2
Calculated molecular weight 53 kDa
Observed molecular weight 54 kDa
GenBank accession numberBC000094
Gene symbol RPS6KB2
Gene ID (NCBI) 6199
RRIDAB_2182250
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RPS6KB2(ribosomal protein S6 kinase beta-2) is also named as 70 kDa ribosomal protein S6 kinase 2(P70S6K2),Serine/threonine-protein kinase 14B(STK14B) and belongs to the protein kinase superfamily. It is activated by mitogenic stimuli, including growth factors, cytokines, and phorbol esters. The activated enzyme phosphorylates several serine residues in the C terminus of ribosomal protein S6(PMID:9878560).

Protocols

Product Specific Protocols
WB protocol for S6K2 antibody 15268-1-APDownload protocol
IHC protocol for S6K2 antibody 15268-1-APDownload protocol
IF protocol for S6K2 antibody 15268-1-APDownload protocol
IP protocol for S6K2 antibody 15268-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Cell

The mTORC1-SLC4A7 axis stimulates bicarbonate import to enhance de novo nucleotide synthesis.

Authors - Eunus S Ali
View Article
IHC

J Ethnopharmacol

Exploring the potential mechanism of Heng-Gu-Gu-Shang-Yu-He-Ji therapy for osteoporosis based on network pharmacology and transcriptomics

Authors - Linbi Xie
View Article