Validation Data Gallery

Filter: AllWBIF/ICC

WB analysis using 18228-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 18228-1-AP (RPS8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 18228-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 18228-1-AP (RPS8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of SGC-7901 using 18228-1-AP
SGC-7901 cells were subjected to SDS PAGE followed by western blot with 18228-1-AP (RPS8 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of A375 using 18228-1-AP
A375 cells were subjected to SDS PAGE followed by western blot with 18228-1-AP (RPS8 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 18228-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 18228-1-AP (RPS8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 18228-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 18228-1-AP (RPS8 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of PC-3 using 18228-1-AP
PC-3 cells were subjected to SDS PAGE followed by western blot with 18228-1-AP (RPS8 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of mouse pancreas using 18228-1-AP
mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 18228-1-AP (RPS8 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IF Staining of A375 using 18228-1-AP
Immunofluorescent analysis of A375 cells using 18228-1-AP (RPS8 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

RPS8 Polyclonal antibody

RPS8 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 18228-1-AP

Publications(7)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Small ribosomal subunit protein eS8, ribosomal protein S8, OK/SW-cl.83, 40S ribosomal protein S8

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Overview

Tested Applications
Product Information
Publications (7)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HeLa cells, mouse liver tissue, SGC-7901 cells, PC-3 cells, mouse pancreas tissue, A375 cells
Positive IF/ICC detected in	A375 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunofluorescence (IF)/ICC	IF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 3 publications below
IHC	See 3 publications below
IF	See 1 publications below

Product Information

18228-1-AP targets RPS8 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	RPS8 fusion protein Ag12313 相同性解析による交差性が予測される生物種
Full Name	ribosomal protein S8
Calculated molecular weight	208 aa, 24 kDa
Observed molecular weight	25-28 kDa
GenBank accession number	BC070875
Gene Symbol	RPS8
Gene ID (NCBI)	6202
RRID	AB_10666168
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P62241
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for RPS8 antibody 18228-1-AP	Download protocol
IF protocol for RPS8 antibody 18228-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	IF	Nat Cell Biol Blebbisomes are large, organelle-rich extracellular vesicles with cell-like properties Authors - Dennis K Jeppesen View Article
mouse	WB	Dev Cell Autophagic elimination of ribosomes during spermiogenesis provides energy for flagellar motility. Authors - Yuqing Lei View Article
human	IHC	Cancer Biol Med A novel recurrence-associated metabolic prognostic model for risk stratification and therapeutic response prediction in patients with stage I lung adenocarcinoma. Authors - Chengming Liu View Article
human	WB	Mol Cell Proteomics Proteomic landscape of exosomes reveals the functional contributions of CD151 in triple-negative breast cancer. Authors - Sipeng Li View Article
human	IHC	Lab Invest Proteins associated with pancreatic cancer survival in patients with resectable pancreatic ductal adenocarcinoma. Authors - Ru Chen View Article
human	IHC	Oncol Rep Identification of 40S ribosomal protein S8 as a novel biomarker for alcohol‑associated hepatocellular carcinoma using weighted gene co‑expression network analysis. Authors - Ningrui Bi View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 18228-1-AP

WB analysis of HeLa using 18228-1-AP

WB analysis of SGC-7901 using 18228-1-AP

WB analysis of A375 using 18228-1-AP

WB analysis of HeLa using 18228-1-AP

WB analysis of mouse liver using 18228-1-AP

WB analysis of PC-3 using 18228-1-AP

WB analysis of mouse pancreas using 18228-1-AP

IF Staining of A375 using 18228-1-AP

RPS8 Polyclonal antibody

RPS8 Polyclonal Antibody for WB, IF/ICC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Blebbisomes are large, organelle-rich extracellular vesicles with cell-like properties

Autophagic elimination of ribosomes during spermiogenesis provides energy for flagellar motility.

A novel recurrence-associated metabolic prognostic model for risk stratification and therapeutic response prediction in patients with stage I lung adenocarcinoma.

Proteomic landscape of exosomes reveals the functional contributions of CD151 in triple-negative breast cancer.

Proteins associated with pancreatic cancer survival in patients with resectable pancreatic ductal adenocarcinoma.

Identification of 40S ribosomal protein S8 as a novel biomarker for alcohol‑associated hepatocellular carcinoma using weighted gene co‑expression network analysis.