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  • Featured Product
  • KD/KO Validated

RPS9 Polyclonal antibody

RPS9 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 18215-1-AP

Synonyms

Small ribosomal subunit protein uS4, ribosomal protein S9, 40S ribosomal protein S9

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse uterus tissue using RPS9 Polyclonal antibody (18215-1-AP)

    WB analysis of mouse uterus using 18215-1-AP

    mouse uterus tissue were subjected to SDS PAGE followed by western blot with 18215-1-AP (RPS9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse ovary tissue using RPS9 Polyclonal antibody (18215-1-AP)

    WB analysis of mouse ovary using 18215-1-AP

    mouse ovary tissue were subjected to SDS PAGE followed by western blot with 18215-1-AP (RPS9 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using RPS9 Polyclonal antibody (18215-1-AP)

    WB analysis of HeLa using 18215-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 18215-1-AP (RPS9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse ovary tissue using RPS9 Polyclonal antibody (18215-1-AP)

    WB analysis of mouse ovary using 18215-1-AP

    mouse ovary tissue were subjected to SDS PAGE followed by western blot with 18215-1-AP (RPS9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse stomach tissue using RPS9 Polyclonal antibody (18215-1-AP)

    IHC staining of mouse stomach using 18215-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 18215-1-AP (RPS9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung tissue using RPS9 Polyclonal antibody (18215-1-AP)

    IHC staining of human lung using 18215-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 18215-1-AP (RPS9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung tissue using RPS9 Polyclonal antibody (18215-1-AP)

    IHC staining of human lung using 18215-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 18215-1-AP (RPS9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse lung tissue using RPS9 Polyclonal antibody (18215-1-AP)

    IHC staining of mouse lung using 18215-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 18215-1-AP (RPS9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using RPS9 Polyclonal antibody (18215-1-AP)

    IHC staining of mouse pancreas using 18215-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 18215-1-AP (RPS9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human testis tissue using RPS9 Polyclonal antibody (18215-1-AP)

    IHC staining of human testis using 18215-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 18215-1-AP (RPS9 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using RPS9 Polyclonal antibody (18215-1-AP)

    IHC staining of human testis using 18215-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 18215-1-AP (RPS9 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using RPS9 Polyclonal antibody (18215-1-AP)

    IHC staining of human skin using 18215-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 18215-1-AP (RPS9 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using RPS9 Polyclonal antibody (18215-1-AP)

    IHC staining of human skin using 18215-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 18215-1-AP (RPS9 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human lung tissue using RPS9 Polyclonal antibody (18215-1-AP)

    IHC staining of human lung using 18215-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 18215-1-AP (RPS9 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung tissue using RPS9 Polyclonal antibody (18215-1-AP)

    IHC staining of human lung using 18215-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 18215-1-AP (RPS9 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using RPS9 Polyclonal antibody (18215-1-AP)

    IHC staining of human ovary using 18215-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 18215-1-AP (RPS9 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using RPS9 Polyclonal antibody (18215-1-AP)

    IHC staining of human ovary using 18215-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 18215-1-AP (RPS9 antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RPS9 Polyclonal antibody (18215-1-AP)

    IF Staining of HeLa using 18215-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 18215-1-AP (RPS9 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inmouse uterus tissue, HeLa cells, mouse ovary tissue
Positive IHC detected inhuman lung tissue, human ovary tissue, human skin tissue, human testis tissue, mouse lung tissue, mouse pancreas tissue, mouse stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18215-1-AP targets RPS9 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RPS9 fusion protein Ag12945 相同性解析による交差性が予測される生物種
Full Name ribosomal protein S9
Calculated molecular weight 23 kDa
Observed molecular weight 25 kDa
GenBank accession numberBC000802
Gene symbol RPS9
Gene ID (NCBI) 6203
RRIDAB_2182390
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RPS9 antibody 18215-1-APDownload protocol
IHC protocol for RPS9 antibody 18215-1-APDownload protocol
IF protocol for RPS9 antibody 18215-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Dev Cell

Autophagic elimination of ribosomes during spermiogenesis provides energy for flagellar motility.

Authors - Yuqing Lei
View Article
humanWB

Proc Natl Acad Sci U S A

Preventing translational inhibition from ribosomal protein insufficiency by a herpes simplex virus-encoded ribosome-associated protein.

Authors - Elizabeth I Vink
  • KD Validated
View Article
humanIHC

Proc Natl Acad Sci U S A

Local translation in nuclear condensate amyloid bodies.

Authors - Phaedra R Theodoridis
View Article
humanWB

Sci Rep

Down-regulation of HSP60 Suppresses the Proliferation of Glioblastoma Cells via the ROS/AMPK/mTOR Pathway.

Authors - Haiping Tang
View Article
humanWB

Front Immunol

Gradient Boosting Machine Learning Model for Defective Endometrial Receptivity Prediction by Macrophage-Endometrium Interaction Modules.

Authors - Bohan Li
View Article
humanWB

Genes (Basel)

Transcriptional Regulation of CD40 Expression by 4 Ribosomal Proteins via a Functional SNP on a Disease-Associated CD40 Locus.

Authors - Meijuan Zou
  • KD Validated
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Wei (Verified Customer) (01-30-2020)

Single, strong band as predicted size 25kDa

  • Applications: Western Blot,
  • Cell Tissue Type: Mouse Heart