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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RPUSD1 Polyclonal antibody (25578-1-AP)

    WB analysis using 25578-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25578-1-AP (RPUSD1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using RPUSD1 Polyclonal antibody (25578-1-AP)

    IP experiment of HeLa using 25578-1-AP

    IP result of anti-RPUSD1 (IP:25578-1-AP, 4ug; Detection:25578-1-AP 1:500) with HeLa cells lysate 1760 ug.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RPUSD1 Polyclonal antibody (25578-1-AP)

    IF Staining of HeLa using 25578-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RPUSD1 antibody (25578-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

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RPUSD1 Polyclonal antibody

RPUSD1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 25578-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, IP, ELISA

Synonyms

RLUCL, C16orf40

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inU2OS cells
Positive IP detected inHeLa cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25578-1-AP targets RPUSD1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RPUSD1 fusion protein Ag22314 相同性解析による交差性が予測される生物種
Full Name RNA pseudouridylate synthase domain containing 1
Calculated molecular weight 312 aa, 35 kDa
Observed molecular weight34~35 kDa
GenBank accession numberBC011783
Gene Symbol RPUSD1
Gene ID (NCBI) 113000
RRIDAB_3669487
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UJJ7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RPUSD1 (RNA pseudouridylate synthase domain-containing protein 1) also known as RLUCL (Ribosomal large subunit pseudouridine synthase C-like protein), was significantly upregulated in HCC and expressed at higher levels in advanced stage or poorly differentiated HCC tissues (PMID: 36437949).

Protocols

Product Specific Protocols
WB protocol for RPUSD1 antibody 25578-1-APDownload protocol
IF protocol for RPUSD1 antibody 25578-1-APDownload protocol
IP protocol for RPUSD1 antibody 25578-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols