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  • Featured Product
  • KD/KO Validated

RPUSD2 Polyclonal antibody

RPUSD2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25707-1-AP

Synonyms

RNA pseudouridylate synthase domain-containing protein 2, Pseudouridylate synthase RPUSD2, EC:5.4.99.-, C18B11, C15orf19

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RPUSD2 Polyclonal antibody (25707-1-AP)

    WB analysis using 25707-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25707-1-AP (RPUSD2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using RPUSD2 Polyclonal antibody (25707-1-AP)

    WB analysis of HeLa using 25707-1-AP

    WB result of RPUSD2 antibody (25707-1-AP; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-RPUSD2 transfected HeLa cells.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using RPUSD2 Polyclonal antibody (25707-1-AP)

    IHC staining of human breast cancer using 25707-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25707-1-AP (RPUSD2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using RPUSD2 Polyclonal antibody (25707-1-AP)

    IHC staining of human colon cancer using 25707-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25707-1-AP (RPUSD2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using RPUSD2 Polyclonal antibody (25707-1-AP)

    IHC staining of human colon cancer using 25707-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25707-1-AP (RPUSD2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using RPUSD2 Polyclonal antibody (25707-1-AP)

    IHC staining of human colon cancer using 25707-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25707-1-AP (RPUSD2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using RPUSD2 Polyclonal antibody (25707-1-AP)

    IHC staining of human colon cancer using 25707-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25707-1-AP (RPUSD2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using RPUSD2 Polyclonal antibody (25707-1-AP)

    IHC staining of human breast cancer using 25707-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25707-1-AP (RPUSD2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells
Positive IHC detected inhuman colon cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25707-1-AP targets RPUSD2 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RPUSD2 fusion protein Ag22452 相同性解析による交差性が予測される生物種
Full Name RNA pseudouridylate synthase domain containing 2
Calculated molecular weight 545 aa, 61 kDa
Observed molecular weight 80-90 kDa
GenBank accession numberBC016967
Gene symbol RPUSD2
Gene ID (NCBI) 27079
RRIDAB_2880202
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RPUSD2 antibody 25707-1-APDownload protocol
IHC protocol for RPUSD2 antibody 25707-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols