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Species Reactivity

Species Reactivity

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  • Featured Product
  • KD/KO Validated

RRAD Polyclonal antibody

RRAD Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 27763-1-AP

Synonyms

Ras associated with diabetes, RAD1, RAD, GTP-binding protein RAD, GTP binding protein RAD

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RRAD Polyclonal antibody (27763-1-AP)

    WB analysis using 27763-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27763-1-AP (RRAD antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SKOV-3 cells using RRAD Polyclonal antibody (27763-1-AP)

    WB analysis of SKOV-3 using 27763-1-AP

    WB result of RRAD antibody (27763-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RRAD transfected SKOV-3 cells.

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Tested Applications

Positive WB detected inmouse heart tissue, SKOV-3 cells, rat heart tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27763-1-AP targets RRAD in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RRAD fusion protein Ag26869 相同性解析による交差性が予測される生物種
Full Name Ras-related associated with diabetes
Calculated molecular weight 33 kDa
Observed molecular weight 29-34 kDa
GenBank accession numberBC057815
Gene symbol RRAD
Gene ID (NCBI) 6236
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RRAD is also named as RAD1. RRAD, a member of the Ras-like small GTPase family, was initially identified as a gene associated with Type II diabetes since it was found to be overexpressed in some Type II diabetic patients (PMID: 8248782). RRAD overexpression reduced insulin-stimulated glucose uptake in cultured muscle and adipocytes cells (PMID: 8798502). RRAD was found to be frequently down-regulated in different types of human cancers, including lung cancer, breast cancer, and nasopharyngeal carcinoma, etc, due to the hypermethylation of its promoter (PMID: 17195088).

Protocols

Product Specific Protocols
WB protocol for RRAD antibody 27763-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols