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  • Featured Product
  • KD/KO Validated

RRAGC Polyclonal antibody

RRAGC Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26989-1-AP

Synonyms

GTPase interacting protein 2, GTR2, Rag C, RAGC, Ras related GTP binding C, RRAGC, TIB929

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using RRAGC Polyclonal antibody (26989-1-AP)

    WB analysis of Jurkat using 26989-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 26989-1-AP (RRAGC Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using RRAGC Polyclonal antibody (26989-1-AP)

    WB analysis of Jurkat using 26989-1-AP

    WB result of RRAGC antibody (26989-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RRAGC transfected Jurkat cells.

  • Western Blot (WB) analysis of HeLa cells using RRAGC Polyclonal antibody (26989-1-AP)

    WB analysis of HeLa using 26989-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 26989-1-AP (RRAGC Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using RRAGC Polyclonal antibody (26989-1-AP)

    WB analysis of MCF-7 using 26989-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 26989-1-AP (RRAGC Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293T cells using RRAGC Polyclonal antibody (26989-1-AP)

    WB analysis of HEK-293T using 26989-1-AP

    HEK-293T cells were subjected to SDS PAGE followed by western blot with 26989-1-AP (RRAGC Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using RRAGC Polyclonal antibody (26989-1-AP)

    WB analysis of NIH/3T3 using 26989-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 26989-1-AP (RRAGC Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inJurkat cells, HeLa cells, MCF-7 cells, HEK-293T cells, NIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 26989-1-AP is RRAGC Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RRAGC fusion protein Ag25780 相同性解析による交差性が予測される生物種
Full Name Ras-related GTP binding C
Calculated molecular weight 44 kDa
Observed molecular weight 44 kDa
GenBank accession numberBC016668
Gene symbol RRAGC
Gene ID (NCBI) 64121
RRIDAB_2880714
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RRAGC antibody 26989-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Autophagy

KAT7-mediated CANX (calnexin) crotonylation regulates leucine-stimulated MTORC1 activity.

Authors - Guokai Yan
View Article
humanWB

Genet Med

De novo missense variants in RRAGC lead to a fatal mTORopathy of early childhood

Authors - Margot R F Reijnders
View Article
human,mouseWB,IHC

Cell Death Dis

Inosine enhances tumor mitochondrial respiration by inducing Rag GTPases and nascent protein synthesis under nutrient starvation

Authors - Mei-Xin Li
View Article