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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RRAGD Polyclonal antibody (15315-1-AP)

    WB analysis using 15315-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15315-1-AP (RRAGD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of NIH/3T3 cells using RRAGD Polyclonal antibody (15315-1-AP)

    IP experiment of NIH/3T3 using 15315-1-AP

    IP result of anti-RRAGD (IP:15315-1-AP, 4ug; Detection:15315-1-AP 1:500) with NIH/3T3 cells lysate 1120 ug.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using RRAGD Polyclonal antibody (15315-1-AP)

    IF Staining of HepG2 using 15315-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RRAGD antibody (15315-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

RRAGD Polyclonal antibody

RRAGD Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 15315-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA

Synonyms

Ras-related GTP-binding protein D, Ras related GTP binding D, RagD, Rag D, EC:3.6.5.-

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inNIH/3T3 cells, mouse skeletal muscle tissue, rat skeletal muscle tissue
Positive IP detected inNIH/3T3 cells
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15315-1-AP targets RRAGD in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RRAGD fusion protein Ag7557 相同性解析による交差性が予測される生物種
Full Name Ras-related GTP binding D
Calculated molecular weight 46 kDa
Observed molecular weight46-50 kDa
GenBank accession numberBC003088
Gene Symbol RRAGD
Gene ID (NCBI) 58528
RRIDAB_3085458
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NQL2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Ras-related GTP-binding protein D (RRAGD) is a monomeric GTP or GDP binding protein, which plays a critical role in mediating amino acid stimulated mTOR signaling pathway, a key pathway in determination of cell growth and proliferation rate. Recently, RRAGD has been reported involving the progression of ovarian cancer and colon cancer.

Protocols

Product Specific Protocols
WB protocol for RRAGD antibody 15315-1-APDownload protocol
IF protocol for RRAGD antibody 15315-1-APDownload protocol
IP protocol for RRAGD antibody 15315-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols