Validation Data Gallery

Filter: AllWBIPIHC

WB analysis using 27457-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 27457-1-AP (RRAS antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 27457-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 27457-1-AP (RRAS antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of PC-3 using 27457-1-AP
PC-3 cells were subjected to SDS PAGE followed by western blot with 27457-1-AP (RRAS antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IP experiment of HT-29 using 27457-1-AP
IP result of anti-RRAS (IP:27457-1-AP, 4ug; Detection:27457-1-AP 1:500) with HT-29 cells lysate 3320 ug.
IHC staining of human renal cell carcinoma using 27457-1-AP
Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 27457-1-AP (RRAS antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human renal cell carcinoma using 27457-1-AP
Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 27457-1-AP (RRAS antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse kidney using 27457-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 27457-1-AP (RRAS antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse colon using 27457-1-AP
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 27457-1-AP (RRAS antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse colon using 27457-1-AP
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 27457-1-AP (RRAS antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human gliomas using 27457-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 27457-1-AP (RRAS antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human gliomas using 27457-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 27457-1-AP (RRAS antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse kidney using 27457-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 27457-1-AP (RRAS antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

RRAS Polyclonal antibody

RRAS Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 27457-1-AP

Publications(5)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, ELISA

Synonyms

p23, Ras related protein R Ras, RRAS

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (5)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	A549 cells, PC-3 cells, HT-29 cells
Positive IP detected in	HT-29 cells
Positive IHC detected in	human renal cell carcinoma tissue, mouse kidney tissue, mouse colon tissue, human gliomas tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 4 publications below
IHC	See 1 publications below

Product Information

27457-1-AP targets RRAS in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	RRAS fusion protein Ag26538 相同性解析による交差性が予測される生物種
Full Name	related RAS viral (r-ras) oncogene homolog
Calculated molecular weight	218 aa, 23 kDa
Observed molecular weight	23 kDa
GenBank accession number	BC016318
Gene Symbol	RRAS
Gene ID (NCBI)	6237
RRID	AB_2880876
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P10301
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for RRAS antibody 27457-1-AP	Download protocol
IHC protocol for RRAS antibody 27457-1-AP	Download protocol
IP protocol for RRAS antibody 27457-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Nat Commun Targeting NRAS via miR-1304-5p or farnesyltransferase inhibition confers sensitivity to ALK inhibitors in ALK-mutant neuroblastoma Authors - Perla Pucci View Article
human，mouse	WB	Biomaterials Polymeric nanoparticle mediated inhibition of miR-21 with enhanced miR-124 expression for combinatorial glioblastoma therapy. Authors - Yuanyuan Liu View Article
human	WB	Transl Cancer Res Identification of RRAS gene related to nasopharyngeal carcinoma based on pathway and network-based analyses. Authors - Ruowen Xiao View Article
human	WB	Hepatology Metabolism-induced tumor activator 1 (MITA1), an Energy Stress-Inducible Long Noncoding RNA, Promotes Hepatocellular Carcinoma Metastasis Authors - Meilin Ma View Article
	IHC	Cancer Lett Integrated multi-omics analyses of oral squamous cell carcinoma reveal precision patient stratification and personalized treatment strategies Authors - Chi-Sheng Wu View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 27457-1-AP

WB analysis of A549 using 27457-1-AP

WB analysis of PC-3 using 27457-1-AP

IP experiment of HT-29 using 27457-1-AP

IHC staining of human renal cell carcinoma using 27457-1-AP

IHC staining of human renal cell carcinoma using 27457-1-AP

IHC staining of mouse kidney using 27457-1-AP

IHC staining of mouse colon using 27457-1-AP

IHC staining of mouse colon using 27457-1-AP

IHC staining of human gliomas using 27457-1-AP

IHC staining of human gliomas using 27457-1-AP

IHC staining of mouse kidney using 27457-1-AP

RRAS Polyclonal antibody

RRAS Polyclonal Antibody for WB, IP, IHC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Targeting NRAS via miR-1304-5p or farnesyltransferase inhibition confers sensitivity to ALK inhibitors in ALK-mutant neuroblastoma

Polymeric nanoparticle mediated inhibition of miR-21 with enhanced miR-124 expression for combinatorial glioblastoma therapy.

Identification of RRAS gene related to nasopharyngeal carcinoma based on pathway and network-based analyses.

Metabolism-induced tumor activator 1 (MITA1), an Energy Stress-Inducible Long Noncoding RNA, Promotes Hepatocellular Carcinoma Metastasis

Integrated multi-omics analyses of oral squamous cell carcinoma reveal precision patient stratification and personalized treatment strategies