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KD/KO Validated

RRBP1 Polyclonal antibody

Name: RRBP1 Polyclonal antibody
Brand: Proteintech
SKU: 22015-1-AP

RRBP1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 22015-1-AP

Synonyms

Ribosome-binding protein 1, ES/130-related protein, ES/130 related protein, 180 kDa ribosome receptor homolog

Validation Data Gallery

Filter:

AllWBIHCIF/ICC

WB analysis using 22015-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 22015-1-AP (RRBP1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
WB analysis of mouse pancreas using 22015-1-AP
mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 22015-1-AP (RRBP1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
IHC staining of human lung cancer using 22015-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22015-1-AP (RRBP1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 22015-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22015-1-AP (RRBP1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 22015-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22015-1-AP (RRBP1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human pancreas using 22015-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 22015-1-AP (RRBP1 Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human pancreas using 22015-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 22015-1-AP (RRBP1 Antibody) at dilution of 1:50 (under 40x lens).
IF Staining of HepG2 using 22015-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RRBP1 antibody (22015-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).
IF Staining of HepG2 using 22015-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RRBP1 antibody (22015-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
IF Staining of NIH/3T3 using 22015-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using RRBP1 antibody (22015-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
IF Staining of NIH/3T3 using 22015-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using RRBP1 antibody (22015-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Cat No. 22015-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HeLa cells, mouse pancreas tissue, HepG2 cells
Positive IHC detected in	human lung cancer tissue, human pancreas tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	NIH/3T3 cells, HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:12000
Immunohistochemistry (IHC)	IHC : 1:2000-1:8000
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 5 publications below
IHC	See 1 publications below
IF	See 3 publications below
IP	See 1 publications below

Product Information

22015-1-AP targets RRBP1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	RRBP1 fusion protein Ag16648 相同性解析による交差性が予測される生物種
Full Name	ribosome binding protein 1 homolog 180kDa (dog)
Calculated molecular weight	1410 aa, 152 kDa
Observed molecular weight	180 kDa
GenBank accession number	BC128577
Gene symbol	RRBP1
Gene ID (NCBI)	6238
RRID	AB_2878971
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Ribosome-binding protein 1 (RRBP1) is an endoplasmic reticulum membrane protein. It is a 1410 amino acid protein composed of a hydrophobic NH2-terminus that includes a transmembrane domain, a highly conserved tandem repeat (ribosome-binding domain), and an acidic coiled-coil COOH-terminal domain. RRBP1 acts as a ribosome receptor and mediates interaction between the ribosome and the endoplasmic reticulum membrane. It is also involved in early cardiac morphogenesis(PMID: 22709790; 8812507; 7686155). RRBP1 has two different isoforms with molecular weights of 150 kDa and 109 kDa.

Protocols

Product Specific Protocols
WB protocol for RRBP1 antibody 22015-1-AP	Download protocol
IHC protocol for RRBP1 antibody 22015-1-AP	Download protocol
IF protocol for RRBP1 antibody 22015-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IF	Sci Adv Oncoprotein SND1 hijacks nascent MHC-I heavy chain to ER-associated degradation, leading to impaired CD8+ T cell response in tumor. Authors - Yuan Wang View Article
human	WB,IF,IP	Mol Oncol USP35 mitigates endoplasmic reticulum stress-induced apoptosis by stabilizing RRBP1 in non-small cell lung cancer. Authors - Wenqing Wang KD Validated View Article
		Acta Biochim Biophys Sin (Shanghai) Thymosin beta 4 silencing suppresses proliferation and invasion of non-small cell lung cancer cells by repressing Notch1 activation. Authors - Dayu Huang View Article
human	WB,IHC	Cancer Manag Res RRBP1 is highly expressed in prostate cancer and correlates with prognosis. Authors - Tieqiu Li View Article
human	WB	Science Endosomal lipid signaling reshapes the endoplasmic reticulum to control mitochondrial function Authors - Wonyul Jang KD Validated View Article
human	WB	Front Oncol RRBP1 depletion of bone metastatic cancer cells contributes to enhanced expression of the osteoblastic phenotype Authors - Rui Chen KD Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RRBP1 Polyclonal antibody

RRBP1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Validation Data Gallery

WB analysis using 22015-1-AP

WB analysis of mouse pancreas using 22015-1-AP

IHC staining of human lung cancer using 22015-1-AP

IHC staining of human lung cancer using 22015-1-AP

IHC staining of human lung cancer using 22015-1-AP

IHC staining of human pancreas using 22015-1-AP

IHC staining of human pancreas using 22015-1-AP

IF Staining of HepG2 using 22015-1-AP

IF Staining of HepG2 using 22015-1-AP

IF Staining of NIH/3T3 using 22015-1-AP

IF Staining of NIH/3T3 using 22015-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Oncoprotein SND1 hijacks nascent MHC-I heavy chain to ER-associated degradation, leading to impaired CD8+ T cell response in tumor.

USP35 mitigates endoplasmic reticulum stress-induced apoptosis by stabilizing RRBP1 in non-small cell lung cancer.

Thymosin beta 4 silencing suppresses proliferation and invasion of non-small cell lung cancer cells by repressing Notch1 activation.

RRBP1 is highly expressed in prostate cancer and correlates with prognosis.

Endosomal lipid signaling reshapes the endoplasmic reticulum to control mitochondrial function

RRBP1 depletion of bone metastatic cancer cells contributes to enhanced expression of the osteoblastic phenotype