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Target

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Species Reactivity

Species Reactivity

Conjugate

Conjugate

RRBP1 Recombinant antibody

RRBP1 Recombinant Antibody for IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230159G12

Cat no : 82890-2-RR

Synonyms

Ribosome-binding protein 1, ES/130-related protein, ES/130 related protein, 230159G12, 180 kDa ribosome receptor homolog

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using RRBP1 Recombinant antibody (82890-2-RR)

    IHC staining of human stomach cancer using 82890-2-RR

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 82890-2-RR (RRBP1 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer(pH9).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using RRBP1 Recombinant antibody (82890-2-RR)

    IF Staining of HepG2 using 82890-2-RR

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using RRBP1 antibody (82890-2-RR, Clone: 230159G12 ) at dilution of 1:500 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR004).

  • Flow cytometry (FC) experiment of A549 cells using RRBP1 Recombinant antibody (82890-2-RR)

    FC experiment of A549 using 82890-2-RR

    1x10^6 A549 cells were intracellularly stained with 0.25 ug Anti-Human RRBP1 (82890-2-RR, Clone:230159G12) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Flow cytometry (FC) experiment of U2OS cells using RRBP1 Recombinant antibody (82890-2-RR)

    FC experiment of U2OS using 82890-2-RR

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug Anti-Human RRBP1 (82890-2-RR, Clone:230159G12) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 82890-2-RR

    Affinity and Kinetic Characterization of 82890-2-RR

    Biolayer interferometry (BLl) kinetic assays of 82890-2-RR against Human RRBP1 were performed. The affinity constant is 1.29 nM.

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Tested Applications

Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inU2OS cells, A549 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82890-2-RR targets RRBP1 in IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen RRBP1 fusion protein Ag16648 相同性解析による交差性が予測される生物種
Full Name ribosome binding protein 1 homolog 180kDa (dog)
Calculated molecular weight 1410 aa, 152 kDa
Observed molecular weight108 kDa, 152 kDa
GenBank accession numberBC128577
Gene symbol RRBP1
Gene ID (NCBI) 6238
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Ribosome-binding protein 1 (RRBP1) is an endoplasmic reticulum membrane protein. It is a 1410 amino acid protein composed of a hydrophobic NH2-terminus that includes a transmembrane domain, a highly conserved tandem repeat (ribosome-binding domain), and an acidic coiled-coil COOH-terminal domain. RRBP1 acts as a ribosome receptor and mediates interaction between the ribosome and the endoplasmic reticulum membrane. It is also involved in early cardiac morphogenesis. (PMID: 22709790; 8812507; 7686155)

Protocols

Product Specific Protocols
IHC protocol for RRBP1 antibody 82890-2-RRDownload protocol
IF protocol for RRBP1 antibody 82890-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols