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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RRBP1 Recombinant antibody

RRBP1 Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230159G10

Cat no : 82890-3-RR

Synonyms

Ribosome-binding protein 1, ES/130-related protein, ES/130 related protein, 230159G10, 180 kDa ribosome receptor homolog

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RRBP1 Recombinant antibody (82890-3-RR)

    WB analysis using 82890-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82890-3-RR (RRBP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using RRBP1 Recombinant antibody (82890-3-RR)

    IHC staining of human stomach cancer using 82890-3-RR

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 82890-3-RR (RRBP1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer(pH9).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RRBP1 Recombinant antibody (82890-3-RR)

    IF Staining of HeLa using 82890-3-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RRBP1 antibody (82890-3-RR, Clone: 230159G10 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HeLa cells using RRBP1 Recombinant antibody (82890-3-RR)

    FC experiment of HeLa using 82890-3-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug RRBP1 Recombinant antibody (82890-3-RR, Clone:230159G10) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Flow cytometry (FC) experiment of U-2 OS using RRBP1 Recombinant antibody (82890-3-RR)

    FC experiment of U-2 OS using 82890-3-RR

    1x10^6 U-2 OS were intracellularly stained with 0.25 ug RRBP1 Recombinant antibody (82890-3-RR, Clone:230159G10) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 82890-3-RR

    Affinity and Kinetic Characterization of 82890-3-RR

    Biolayer interferometry (BLl) kinetic assays of 82890-3-RR against Human RRBP1 were performed. The affinity constant is 3.26 nM.

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Tested Applications

Positive WB detected inHeLa cells, NCI-H1299 cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells, U-2 OS

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 82890-3-RR is RRBP1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen RRBP1 fusion protein Ag16648 相同性解析による交差性が予測される生物種
Full Name ribosome binding protein 1 homolog 180kDa (dog)
Calculated molecular weight 1410 aa, 152 kDa
Observed molecular weight180 kDa
GenBank accession numberBC128577
Gene symbol RRBP1
Gene ID (NCBI) 6238
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Ribosome-binding protein 1 (RRBP1) is an endoplasmic reticulum membrane protein. It is a 1410 amino acid protein composed of a hydrophobic NH2-terminus that includes a transmembrane domain, a highly conserved tandem repeat (ribosome-binding domain), and an acidic coiled-coil COOH-terminal domain. RRBP1 acts as a ribosome receptor and mediates interaction between the ribosome and the endoplasmic reticulum membrane. It is also involved in early cardiac morphogenesis(PMID: 22709790; 8812507; 7686155). RRBP1 has two different isoforms with molecular weights of 150 kDa and 109 kDa.

Protocols

Product Specific Protocols
WB protocol for RRBP1 antibody 82890-3-RRDownload protocol
IHC protocol for RRBP1 antibody 82890-3-RRDownload protocol
IF protocol for RRBP1 antibody 82890-3-RRDownload protocol
FC protocol for RRBP1 antibody 82890-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols