Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, NCI-H1299 cells |
| Positive IHC detected in | human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
| Positive FC (Intra) detected in | HeLa cells, U-2 OS |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:125-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
82890-3-RR targets RRBP1 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | RRBP1 fusion protein Ag16648 相同性解析による交差性が予測される生物種 |
| Full Name | ribosome binding protein 1 homolog 180kDa (dog) |
| Calculated molecular weight | 1410 aa, 152 kDa |
| Observed molecular weight | 180 kDa |
| GenBank accession number | BC128577 |
| Gene Symbol | RRBP1 |
| Gene ID (NCBI) | 6238 |
| RRID | AB_3670616 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purfication |
| UNIPROT ID | Q9P2E9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Ribosome-binding protein 1 (RRBP1) is an endoplasmic reticulum membrane protein. It is a 1410 amino acid protein composed of a hydrophobic NH2-terminus that includes a transmembrane domain, a highly conserved tandem repeat (ribosome-binding domain), and an acidic coiled-coil COOH-terminal domain. RRBP1 acts as a ribosome receptor and mediates interaction between the ribosome and the endoplasmic reticulum membrane. It is also involved in early cardiac morphogenesis(PMID: 22709790; 8812507; 7686155). RRBP1 has two different isoforms with molecular weights of 150 kDa and 109 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RRBP1 antibody 82890-3-RR | Download protocol |
| IHC protocol for RRBP1 antibody 82890-3-RR | Download protocol |
| IF protocol for RRBP1 antibody 82890-3-RR | Download protocol |
| FC protocol for RRBP1 antibody 82890-3-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |