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KD/KO Validated

RRM1 Polyclonal antibody

Name: RRM1 Polyclonal antibody
Brand: Proteintech
SKU: 10526-1-AP

RRM1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, monkey and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 10526-1-AP

Synonyms

RR1, RIR1, ribonucleotide reductase M1, Ribonucleoside-diphosphate reductase subunit M1, Ribonucleoside-diphosphate reductase large subunit

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICCFC (Intra)

WB analysis using 10526-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 10526-1-AP (RRM1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 10526-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 10526-1-AP (RRM1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 10526-1-AP
A549 cells (shcontrol and shRNA of RRM1) were subjected to SDS PAGE followed by western blot with 10526-1-AP (RRM1 antibody) at dilution of 1:2000. .
WB analysis of K-562 using 10526-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 10526-1-AP (RRM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of BxPC-3 using 10526-1-AP
BxPC-3 cells were subjected to SDS PAGE followed by western blot with 10526-1-AP (RRM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 10526-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 10526-1-AP (RRM1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of rat skeletal muscle using 10526-1-AP
rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10526-1-AP (RRM1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of K-562 using 10526-1-AP
IP result of anti-RRM1 (IP:10526-1-AP, 5ug; Detection:10526-1-AP 1:800) with K-562 cells lysate 8000ug.
IHC staining of human breast cancer using 10526-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10526-1-AP (RRM1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 10526-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10526-1-AP (RRM1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 10526-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10526-1-AP (RRM1 Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human lung cancer using 10526-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10526-1-AP (RRM1 Antibody) at dilution of 1:200 (under 40x lens).
IF Staining of HepG2 using 10526-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RRM1 antibody (10526-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).
IF Staining of Raji using 10526-1-AP
Immunofluorescent analysis of (4% PFA) fixed Raji cells using RRM1 antibody (10526-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
FC experiment of HeLa using 10526-1-AP
1x10^6 HeLa cells were intracellularly stained with 0.4 ug RRM1 Polyclonal antibody (10526-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Cat No. 10526-1-AP

Size/Concentration

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	A431 cells, HeLa cells, A549 cells, K-562 cells, BxPC-3 cells, HEK-293 cells, U-251 cells
Positive IP detected in	K-562 cells
Positive IHC detected in	human breast cancer tissue, human lung cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells, Raji cells
Positive FC (Intra) detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:16000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:100-1:600
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 14 publications below
IHC	See 33 publications below
IF	See 2 publications below
IP	See 1 publications below

Product Information

10526-1-AP targets RRM1 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.

Tested Reactivity	human, mouse, rat, monkey
Cited Reactivity	human, mouse, xenopus
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	RRM1 fusion protein Ag0789 相同性解析による交差性が予測される生物種
Full Name	ribonucleotide reductase M1
Calculated molecular weight	90 kDa
Observed molecular weight	90 kDa
GenBank accession number	BC006498
Gene symbol	RRM1
Gene ID (NCBI)	6240
RRID	AB_2180372
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Ribonucleoside-diphosphate reductase large subunit (RRM1) is the main enzyme for de novo deoxyribonucleotide

synthesis necessary for DNA synthesis during the cell cycle as well as during DNA repair.

What is the molecular weight of RRM1?

The molecular weight of RRM1 is 90 kDa. RRM1 is a part of ribonucleotide reductase (RNR), which is composed

of two large RRM1 and two small RRM2 subunits in S phase and two large RRM1 and two alternative small p53R2

subunits in non-dividing quiescent cells (PMID: 17416930).

What is the subcellular localization of RRM1?

RRM1 localizes to the cytoplasm, where it is responsible for dNTP production, forming a complex with RRM2.

However, RRM1 can be recruited to DNA damage sites in the nucleus via interaction with Tip60 (PMID: 20159953).

What is the tissue expression pattern of RRM1?

RRM1 is ubiquitously expressed.

How is RRM1 expression regulated during the cell cycle and upon DNA damage?

Rrm1 gene expression levels depend on the cell cycle, and the highest mRNA levels are observed in S phase.

However, RRM1 protein levels are constant throughout the cell cycle due to long protein half-life, contrary to

RRM2, which is actively degraded after cell exit from S phase. During DNA damage, e.g., induced with genotoxins

or UV light, expression of RRM1 and RRM2/p53R2 is upregulated (PMID: 1551913 and 8798592).

Protocols

Product Specific Protocols
WB protocol for RRM1 antibody 10526-1-AP	Download protocol
IHC protocol for RRM1 antibody 10526-1-AP	Download protocol
IF protocol for RRM1 antibody 10526-1-AP	Download protocol
IP protocol for RRM1 antibody 10526-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB, IF	N Engl J Med DNA synthesis and repair genes RRM1 and ERCC1 in lung cancer. Authors - Zhong Zheng View Article
human	IF	Lancet Oncol The ribonucleotide reductase large subunit (RRM1) as a predictive factor in patients with cancer. Authors - Lars Petter Jordheim View Article
human	WB	Clin Cancer Res PMID: 25224279 Authors - Martin Kerr KD Validated View Article
human	WB, IHC, IP	Clin Cancer Res PMID: 26228206 Authors - Francesca Vena View Article
human	IHC	Ann Oncol PMID: 23038758 Authors - Vilmar A C AC View Article
	IHC	Oncotarget Impact of molecular profiling on overall survival of patients with advanced ovarian cancer. Authors - Thomas J Herzog View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RRM1 Polyclonal antibody

RRM1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Validation Data Gallery

WB analysis using 10526-1-AP

WB analysis of HeLa using 10526-1-AP

WB analysis of A549 using 10526-1-AP

WB analysis of K-562 using 10526-1-AP

WB analysis of BxPC-3 using 10526-1-AP

WB analysis of A549 using 10526-1-AP

WB analysis of rat skeletal muscle using 10526-1-AP

IP experiment of K-562 using 10526-1-AP

IHC staining of human breast cancer using 10526-1-AP

IHC staining of human breast cancer using 10526-1-AP

IHC staining of human lung cancer using 10526-1-AP

IHC staining of human lung cancer using 10526-1-AP

IF Staining of HepG2 using 10526-1-AP

IF Staining of Raji using 10526-1-AP

FC experiment of HeLa using 10526-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

DNA synthesis and repair genes RRM1 and ERCC1 in lung cancer.

The ribonucleotide reductase large subunit (RRM1) as a predictive factor in patients with cancer.

PMID: 25224279

PMID: 26228206

PMID: 23038758

Impact of molecular profiling on overall survival of patients with advanced ovarian cancer.

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