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Validation Data Gallery

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  • Western Blot (WB) analysis of K-562 cells using RRM1 Monoclonal antibody (60073-2-Ig)

    WB analysis of K-562 using 60073-2-Ig (same clone as 60073-2-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 60073-2-Ig (RRM1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60073-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using RRM1 Monoclonal antibody (60073-2-Ig)

    WB analysis of HeLa using 60073-2-Ig (same clone as 60073-2-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 60073-2-Ig (RRM1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60073-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of K-562 cells using RRM1 Monoclonal antibody (60073-2-Ig)

    WB analysis of K-562 using 60073-2-Ig (same clone as 60073-2-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 60073-2-Ig (RRM1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60073-2-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of K-562 cells using RRM1 Monoclonal antibody (60073-2-Ig)

    IP experiment of K-562 using 60073-2-Ig (same clone as 60073-2-PBS)

    IP result of anti-RRM1 (IP:60073-2-Ig, 5ug; Detection:60073-2-Ig 1:1000) with K-562 cells lysate 3440ug. This data was developed using the same antibody clone with 60073-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using RRM1 Monoclonal antibody (60073-2-Ig)

    IHC staining of human breast cancer using 60073-2-Ig (same clone as 60073-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60073-2-Ig (RRM1 antibody) at dilution of 1:5000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60073-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using RRM1 Monoclonal antibody (60073-2-Ig)

    IHC staining of human breast cancer using 60073-2-Ig (same clone as 60073-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60073-2-Ig (RRM1 antibody) at dilution of 1:5000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60073-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using RRM1 Monoclonal antibody (60073-2-Ig)

    IHC staining of human pancreas cancer using 60073-2-Ig (same clone as 60073-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 60073-2-Ig (RRM1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60073-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human urothelial carcinoma tissue using RRM1 Monoclonal antibody (60073-2-Ig)

    IHC staining of human urothelial carcinoma using 60073-2-Ig (same clone as 60073-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 60073-2-Ig (RRM1 antibody) at dilution of 1:5000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60073-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human urothelial carcinoma tissue using RRM1 Monoclonal antibody (60073-2-Ig)

    IHC staining of human urothelial carcinoma using 60073-2-Ig (same clone as 60073-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 60073-2-Ig (RRM1 antibody) at dilution of 1:5000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60073-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using RRM1 Monoclonal antibody (60073-2-Ig)

    IHC staining of human lung cancer using 60073-2-Ig (same clone as 60073-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60073-2-Ig (RRM1 antibody) at dilution of 1:5000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60073-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using RRM1 Monoclonal antibody (60073-2-Ig)

    IHC staining of human lung cancer using 60073-2-Ig (same clone as 60073-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60073-2-Ig (RRM1 antibody) at dilution of 1:5000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60073-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using RRM1 Monoclonal antibody (60073-2-Ig)

    IHC staining of human pancreas cancer using 60073-2-Ig (same clone as 60073-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 60073-2-Ig (RRM1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60073-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using RRM1 Monoclonal antibody (60073-2-Ig)

    IHC staining of human pancreas cancer using 60073-2-Ig (same clone as 60073-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 60073-2-Ig (RRM1 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60073-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using RRM1 Monoclonal antibody (60073-2-Ig)

    IHC staining of human colon cancer using 60073-2-Ig (same clone as 60073-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60073-2-Ig (RRM1 antibody) at dilution of 1:5000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60073-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using RRM1 Monoclonal antibody (60073-2-Ig)

    IHC staining of human colon cancer using 60073-2-Ig (same clone as 60073-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60073-2-Ig (RRM1 antibody) at dilution of 1:5000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60073-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human breast cancer tissue using RRM1 Monoclonal antibody (60073-2-Ig)

    IF Staining of human breast cancer using 60073-2-Ig (same clone as 60073-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using RRM1 antibody (60073-2-Ig, Clone: 5H6F3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60073-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using RRM1 Monoclonal antibody (60073-2-Ig)

    IF Staining of HepG2 using 60073-2-Ig (same clone as 60073-2-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using RRM1 antibody (60073-2-Ig, Clone: 5H6F3 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60073-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RRM1 Monoclonal antibody (60073-2-Ig)

    IF Staining of HeLa using 60073-2-Ig (same clone as 60073-2-PBS)

    Immunofluorescent analysis of HeLa cells using 60073-2-Ig (RRM1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L). This data was developed using the same antibody clone with 60073-2-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

RRM1 Monoclonal antibody, PBS Only

RRM1 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA
Cat No. 60073-2-PBS
Clone No.5H6F3

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA

Synonyms

RIR1, ribonucleotide reductase M1, Ribonucleoside-diphosphate reductase subunit M1, Ribonucleoside-diphosphate reductase large subunit, EC:1.17.4.1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60073-2-PBS targets RRM1 in WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen RRM1 fusion protein Ag0789 相同性解析による交差性が予測される生物種
Full Name ribonucleotide reductase M1
Calculated molecular weight 90 kDa
Observed molecular weight 90 kDa
GenBank accession numberBC006498
Gene Symbol RRM1
Gene ID (NCBI) 6240
RRIDAB_10597538
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP23921
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Ribonucleoside-diphosphate reductase functions as a heterodimer of a large and a small subunits in deoxyribonucleotide synthesis. RRM1 constitutes to the large subunit (R1) of ribonucleotide reductase, and it can either form heterodimer with small subunit RRM or RRM2B(PMID:16376858). RRM1 provides the precursors necessary for DNA synthesis. RRM1 can not be detected in quiescent cells, while its mRNA and protein are present throughout the cell cycle in cycling cells(PMID:8188248). Researches showed that RRM1 is involved in carcinogenesis, tumor progression, and the resistance of non-small-cell lung cancer (NSCLC) to treatment. Low level expression of RRM1 in NSCLC is associated with poor survival(PMID:17314339).