☆ 3/31(月)まで!二次抗体 & FlexAbleが30%オフ! >> 免疫アッセイサポートキャンペーン

RRM2 Polyclonal antibody

RRM2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Cat No. 11661-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, RIP, ELISA

RR2M, RR2, R2, EC:1.17.4.1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -


ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。


国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inHeLa cells, A431 cells, HUVEC cells, K-562 cells
Positive IP detected inK-562 cells
Positive IHC detected inhuman appendicitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11661-1-AP targets RRM2 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, RIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, chicken
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RRM2 fusion protein Ag2203 相同性解析による交差性が予測される生物種
Full Name ribonucleotide reductase M2 polypeptide
Calculated molecular weight 389 aa, 45 kDa
Observed molecular weight 45 kDa
GenBank accession numberBC030154
Gene Symbol RRM2
Gene ID (NCBI) 6241
RRIDAB_2180392
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP31350
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Ribonucleotide reductase M2 subunit is one of two subunits that constitute ribonucleotide reductase, the enzyme that catalyzes the conversion of ribonucleotide 5'-diphosphates into 2'-deoxyribonucleotides, a rate-limiting step in the production of 2'-deoxyribonucleoside 5'-diphosphates (dNTP) required for DNA synthesis and repair that is required for DNA synthesis and repair [PMID:20825972, 19250552]. RRM2 is only expressed during the late G1/early S phase, and degraded in late S phase, and the activity of RNR, and therefore DNA synthesis and cell proliferation, is controlled during the cell cycle by the synthesis and degradation of RRM2 subunit [PMID:3894352].

Protocols

Product Specific Protocols
WB protocol for RRM2 antibody 11661-1-APDownload protocol
IHC protocol for RRM2 antibody 11661-1-APDownload protocol
IF protocol for RRM2 antibody 11661-1-APDownload protocol
IP protocol for RRM2 antibody 11661-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Cell Rep

The mechano-chemical circuit in fibroblasts and dendritic cells drives basal cell proliferation in psoriasis

Authors - Jingwei Jiang
humanWB

Oxid Med Cell Longev

lncRNA IGF2-AS Regulates Nucleotide Metabolism by Mediating HMGA1 to Promote Pyroptosis of Endothelial Progenitor Cells in Sepsis Patients.

Authors - Guibin Liang
humanIHC

Front Cell Dev Biol

A Novel Ferroptosis-Based Molecular Signature Associated with Biochemical Recurrence-Free Survival and Tumor Immune Microenvironment of Prostate Cancer.

Authors - Zhi-Bin Ke
WB

Antiviral Res

Long-term HIV-1 infection induces an antiviral state in primary macrophages.

Authors - Maria Pujantell
humanIHC

Front Oncol

Novel Prognostic Signatures of Hepatocellular Carcinoma Based on Metabolic Pathway Phenotypes.

Authors - Tingbo Ye
humanWB

Biomed Pharmacother

Identification of osalmid metabolic profile and active metabolites with anti-tumor activity in human hepatocellular carcinoma cells.

Authors - Zhe Wu
Loading...