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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RRM2 Monoclonal antibody, PBS Only

RRM2 Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

CloneNo.

2A9A7

Cat no : 67006-1-PBS

Synonyms

RR2M, RR2, R2, EC:1.17.4.1, 2A9A7

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RRM2 Monoclonal antibody (67006-1-Ig)

    WB analysis using 67006-1-Ig (same clone as 67006-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67006-1-Ig (RRM2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67006-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using RRM2 Monoclonal antibody (67006-1-Ig)

    WB analysis of HEK-293 using 67006-1-Ig (same clone as 67006-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 67006-1-Ig (RRM2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67006-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using RRM2 Monoclonal antibody (67006-1-Ig)

    IP experiment of HEK-293 using 67006-1-Ig (same clone as 67006-1-PBS)

    IP result of anti-RRM2 (IP:67006-1-Ig, 4ug; Detection:67006-1-Ig 1:2000) with HEK-293 cells lysate 1470 ug. This data was developed using the same antibody clone with 67006-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using RRM2 Monoclonal antibody (67006-1-Ig)

    IHC staining of human skin cancer using 67006-1-Ig (same clone as 67006-1-PBS)

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 67006-1-Ig (RRM2 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67006-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using RRM2 Monoclonal antibody (67006-1-Ig)

    IF Staining of HepG2 using 67006-1-Ig (same clone as 67006-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 67006-1-Ig (RRM2 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67006-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67006-1-PBS targets RRM2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RRM2 fusion protein Ag28664 相同性解析による交差性が予測される生物種
Full Name ribonucleotide reductase M2 polypeptide
Calculated molecular weight 389 aa, 45 kDa
Observed molecular weight 45 kDa
GenBank accession numberBC030154
Gene symbol RRM2
Gene ID (NCBI) 6241
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Ribonucleotide reductase M2 subunit is one of two subunits that constitute ribonucleotide reductase, the enzyme that catalyzes the conversion of ribonucleotide 5'-diphosphates into 2'-deoxyribonucleotides, a rate-limiting step in the production of 2'-deoxyribonucleoside 5'-diphosphates (dNTP) required for DNA synthesis and repair that is required for DNA synthesis and repair [PMID:20825972, 19250552]. RRM2 is only expressed during the late G1/early S phase, and degraded in late S phase, and the activity of RNR, and therefore DNA synthesis and cell proliferation, is controlled during the cell cycle by the synthesis and degradation of RRM2 subunit [PMID:3894352].