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  • Featured Product
  • KD/KO Validated

RRP1 Polyclonal antibody

RRP1 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 14896-1-AP

Synonyms

Novel nuclear protein 1, NOP52, NNP-1, NNP1, NNP 1

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using RRP1 Polyclonal antibody (14896-1-AP)

    WB analysis of HEK-293 using 14896-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 14896-1-AP (RRP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using RRP1 Polyclonal antibody (14896-1-AP)

    WB analysis of HeLa using 14896-1-AP

    WB result of RRP1 antibody (14896-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RRP1 transfected HeLa cells.

  • Western Blot (WB) analysis of human spleen tissue using RRP1 Polyclonal antibody (14896-1-AP)

    WB analysis of human spleen using 14896-1-AP

    human spleen tissue were subjected to SDS PAGE followed by western blot with 14896-1-AP (RRP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using RRP1 Polyclonal antibody (14896-1-AP)

    WB analysis of HEK-293 using 14896-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 14896-1-AP (RRP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using RRP1 Polyclonal antibody (14896-1-AP)

    WB analysis of HeLa using 14896-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 14896-1-AP (RRP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RRP1 Polyclonal antibody (14896-1-AP)

    IF Staining of HeLa using 14896-1-AP

    Immunofluorescent analysis of HeLa cells using 14896-1-AP (RRP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, human spleen tissue
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14896-1-AP targets RRP1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RRP1 fusion protein Ag6686 相同性解析による交差性が予測される生物種
Full Name ribosomal RNA processing 1 homolog (S. cerevisiae)
Calculated molecular weight 53 kDa
Observed molecular weight 53 kDa
GenBank accession numberBC000380
Gene symbol RRP1
Gene ID (NCBI) 8568
RRIDAB_2180625
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RRP1 antibody 14896-1-APDownload protocol
IF protocol for RRP1 antibody 14896-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols