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Species Reactivity
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Species Reactivity
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RRP12 Polyclonal antibody

RRP12 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 26849-1-AP

Synonyms

KIAA0690, RRP12, RRP12 like protein

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RRP12 Polyclonal antibody (26849-1-AP)

    WB analysis using 26849-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26849-1-AP (RRP12 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RRP12 Polyclonal antibody (26849-1-AP)

    IF Staining of HeLa using 26849-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RRP12 antibody (26849-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:100000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26849-1-AP targets RRP12 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RRP12 fusion protein Ag25271 相同性解析による交差性が予測される生物種
Full Name ribosomal RNA processing 12 homolog (S. cerevisiae)
Calculated molecular weight 1297 aa, 144 kDa
Observed molecular weight144-150 kDa
GenBank accession numberBC012745
Gene symbol RRP12
Gene ID (NCBI) 23223
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RRP12 antibody 26849-1-APDownload protocol
IF protocol for RRP12 antibody 26849-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols