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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RRP1B Polyclonal antibody

RRP1B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 27723-1-AP

Synonyms

RRP1-like protein B, RRP1 like protein B, Ribosomal RNA processing protein 1 homolog B

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using RRP1B Polyclonal antibody (27723-1-AP)

    WB analysis of HEK-293 using 27723-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27723-1-AP (RRP1B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using RRP1B Polyclonal antibody (27723-1-AP)

    WB analysis of HepG2 using 27723-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 27723-1-AP (RRP1B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using RRP1B Polyclonal antibody (27723-1-AP)

    WB analysis of HeLa using 27723-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 27723-1-AP (RRP1B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using RRP1B Polyclonal antibody (27723-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 27723-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 27723-1-AP (RRP1B antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using RRP1B Polyclonal antibody (27723-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 27723-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 27723-1-AP (RRP1B antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using RRP1B Polyclonal antibody (27723-1-AP)

    IHC staining of human ovary cancer using 27723-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 27723-1-AP (RRP1B antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using RRP1B Polyclonal antibody (27723-1-AP)

    IHC staining of human ovary cancer using 27723-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 27723-1-AP (RRP1B antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using RRP1B Polyclonal antibody (27723-1-AP)

    IF Staining of A431 using 27723-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using 27723-1-AP (RRP1B antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHEK-293 cells, HepG2 cells, HeLa cells, K-562 cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue, human ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27723-1-AP targets RRP1B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RRP1B fusion protein Ag23405 相同性解析による交差性が予測される生物種
Full Name ribosomal RNA processing 1 homolog B (S. cerevisiae)
Observed molecular weight 84 kDa
GenBank accession numberBC028386
Gene symbol RRP1B
Gene ID (NCBI) 23076
RRIDAB_2880953
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Ribosomal RNA Processing 1 Homolog B (RRP1B) is a protein that has been implicated in several biological processes, particularly in the context of cancer progression and viral replication. RRP1B was first identified as a metastasis susceptibility gene in breast cancer through its ability to modulate gene expression in a manner that can be used to accurately predict prognosis in breast cancer . It has been shown to interact with the metastasis modifier gene, Sipa1, and modulate elements of metastasis predictive gene expression signatures.

Protocols

Product Specific Protocols
WB protocol for RRP1B antibody 27723-1-APDownload protocol
IHC protocol for RRP1B antibody 27723-1-APDownload protocol
IF protocol for RRP1B antibody 27723-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols