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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RRP7A Polyclonal antibody

RRP7A Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 24490-1-AP

Synonyms

BK126B4.3, CGI 96, Gastric cancer antigen Zg14, RRP7A

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse thymus tissue using RRP7A Polyclonal antibody (24490-1-AP)

    WB analysis of mouse thymus using 24490-1-AP

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 24490-1-AP (RRP7A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human brain tissue using RRP7A Polyclonal antibody (24490-1-AP)

    IHC staining of human brain using 24490-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 24490-1-AP (RRP7A Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using RRP7A Polyclonal antibody (24490-1-AP)

    IHC staining of human stomach cancer using 24490-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 24490-1-AP (RRP7A Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using RRP7A Polyclonal antibody (24490-1-AP)

    IHC staining of human stomach cancer using 24490-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 24490-1-AP (RRP7A Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using RRP7A Polyclonal antibody (24490-1-AP)

    IHC staining of human brain using 24490-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 24490-1-AP (RRP7A Antibody) at dilution of 1:50 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using RRP7A Polyclonal antibody (24490-1-AP)

    IF Staining of A431 using 24490-1-AP

    Immunofluorescent analysis of A431 cells using 24490-1-AP (RRP7A antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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Tested Applications

Positive WB detected inmouse thymus tissue
Positive IHC detected inhuman brain tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 24490-1-AP is RRP7A Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RRP7A fusion protein Ag21636 相同性解析による交差性が予測される生物種
Full Name ribosomal RNA processing 7 homolog A (S. cerevisiae)
Calculated molecular weight 280 aa, 32 kDa
Observed molecular weight 42 kDa
GenBank accession numberBC121118
Gene symbol RRP7A
Gene ID (NCBI) 27341
RRIDAB_2879570
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RRP7A, also named as Ribosomal RNA-processing protein 7 homolog A, is a 280 amino acid protein, which belongs to the RRP7 family. The calcualted molecular weight of RRP7A is 32 kDa, but modified RRP7A is about 42 kDa.

Protocols

Product Specific Protocols
WB protocol for RRP7A antibody 24490-1-APDownload protocol
IHC protocol for RRP7A antibody 24490-1-APDownload protocol
IF protocol for RRP7A antibody 24490-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols