Validation Data Gallery

Filter: AllWBIP

WB analysis of K-562 using 15329-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 15329-1-AP (RRS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis using 15329-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15329-1-AP (RRS1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 15329-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 15329-1-AP (RRS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of rat liver using 15329-1-AP
rat liver tissue were subjected to SDS PAGE followed by western blot with 15329-1-AP (RRS1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IP experiment of K-562 using 15329-1-AP
IP result of anti-RRS1 (IP:15329-1-AP, 4ug; Detection:15329-1-AP 1:800) with K-562 cells lysate 3200ug.

Featured Product
KD/KO Validated

RRS1 Polyclonal antibody

RRS1 Polyclonal Antibody for WB, IP, ELISA

Cat No. 15329-1-AP

Publications(5)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Synonyms

KIAA0112, RRR

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (5)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	K-562 cells, HeLa cells
Positive IP detected in	K-562 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 5 publications below

Product Information

15329-1-AP targets RRS1 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag7030 Product name: Recombinant human RRS1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-365 aa of BC001811 Sequence: MEGQSVEELLAKAEQDEAEKLQRITVHKELELQFDLGNLLASDRNPPTGLRCAGPTPEAELQALARDNTQLLINQLWQLPTERVEEAIVARLPEPTTRLPREKPLPRPRPLTRWQQFARLKGIRPKKKTNLVWDEVSGQWRRRWGYQRARDDTKEWLIEVPGNADPLEDQFAKRIQAKKERVAKNELNRLRNLARAHKMQLPSAAGLHPTGHQSKEELGRAMQVAKVSTASVGRFQERLPKEKVPRGSGKKRKFQPLFGDFAAEKKNQLELLRVMNSKKPQLDVTRATNKQMREEDQEEAAKRRKMSQKGKRKGGRQGPGGKRKGGPPSQGGKRKGGLGGKMNSGPPGLGGKRKGGQRPGGKRRK 相同性解析による交差性が予測される生物種
Full Name	RRS1 ribosome biogenesis regulator homolog (S. cerevisiae)
Calculated molecular weight	41 kDa
Observed molecular weight	41 kDa
GenBank accession number	BC001811
Gene Symbol	RRS1
Gene ID (NCBI)	23212
RRID	AB_2301118
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q15050
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
IP protocol for RRS1 antibody 15329-1-AP	Download protocol
WB protocol for RRS1 antibody 15329-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Sci Adv Genomic gain of RRS1 promotes hepatocellular carcinoma through reducing the RPL11-MDM2-p53 signaling. Authors - Pengbo Cao KD Validated View Article
human	WB	Nat Commun Targeting dual-specificity tyrosine phosphorylation-regulated kinase 2 with a highly selective inhibitor for the treatment of prostate cancer. Authors - Kai Yuan View Article
human	WB	Biol Direct Depletion of LONP2 unmasks differential requirements for peroxisomal function between cell types and in cholesterol metabolism Authors - Akihiro Yamashita View Article
human	WB	Tissue Cell Silencing ribosome biogenesis regulator 1 homolog (RRS1) inhibits angiogenesis and cisplatin resistance of lung cancer cells by activating ferroptosis mediated by p53 pathway Authors - Ling Lin View Article
mouse	WB	Adv Sci (Weinh) m6A Reader hnRNPA2B1 Modulates Late Pachytene Progression in Male Meiosis Through Post-Transcriptional Control Authors - Lisha Yin View Article

製品

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企業情報

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Target

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Validation Data Gallery

WB analysis of K-562 using 15329-1-AP

WB analysis using 15329-1-AP

WB analysis of HeLa using 15329-1-AP

WB analysis of rat liver using 15329-1-AP

IP experiment of K-562 using 15329-1-AP

RRS1 Polyclonal antibody

RRS1 Polyclonal Antibody for WB, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Genomic gain of RRS1 promotes hepatocellular carcinoma through reducing the RPL11-MDM2-p53 signaling.

Targeting dual-specificity tyrosine phosphorylation-regulated kinase 2 with a highly selective inhibitor for the treatment of prostate cancer.

Depletion of LONP2 unmasks differential requirements for peroxisomal function between cell types and in cholesterol metabolism

Silencing ribosome biogenesis regulator 1 homolog (RRS1) inhibits angiogenesis and cisplatin resistance of lung cancer cells by activating ferroptosis mediated by p53 pathway

m6A Reader hnRNPA2B1 Modulates Late Pachytene Progression in Male Meiosis Through Post-Transcriptional Control